Aim of present study was to evaluate any possible estrogenic effect induced by 4-nonylphenol in Shubunkin (Carassius auratus) by quantifying plasmatic concentration of the precursor of yolk proteins, vitellogenin (VTG). Adult male Carassius auratus were intraperitoneally administered 4-nonylphenol (10 and 25 mg/kg), vehicle (ethanol 2% in saline) and estradiol 17- (5 mg/kg) and VTG plasma levels were determined by ELISA at 10 and 20 days post treatment. Already at day 10 post-treatment estradiol 17- and 4-nonylphenol at 25 mg/kg induce vitellogenin production, while no effect was observed for vehicle and 4-nonylphenol at 10 mg/kg. Obtained data confirm the estrogenic activity exerted by nonylphenol in teleost species and the reliability of Carassius auratus, Shubunkin variety, as testing species for endocrine disruption effects evaluation.

Studio preliminare sull’effetto estrogenico del 4-nonilfenolo somministrato per via intraperitoneale a maschi adulti di Shubunkin (Carassius auratus, L.).

ZACCARONI, ANNALISA;GAMBERONI, MATTEO;MORDENTI, OLIVIERO;PARMEGGIANI, ALBAMARIA
2006

Abstract

Aim of present study was to evaluate any possible estrogenic effect induced by 4-nonylphenol in Shubunkin (Carassius auratus) by quantifying plasmatic concentration of the precursor of yolk proteins, vitellogenin (VTG). Adult male Carassius auratus were intraperitoneally administered 4-nonylphenol (10 and 25 mg/kg), vehicle (ethanol 2% in saline) and estradiol 17- (5 mg/kg) and VTG plasma levels were determined by ELISA at 10 and 20 days post treatment. Already at day 10 post-treatment estradiol 17- and 4-nonylphenol at 25 mg/kg induce vitellogenin production, while no effect was observed for vehicle and 4-nonylphenol at 10 mg/kg. Obtained data confirm the estrogenic activity exerted by nonylphenol in teleost species and the reliability of Carassius auratus, Shubunkin variety, as testing species for endocrine disruption effects evaluation.
Atti LX Convegno della Società Italiana delle Scienze Veterinarie, Terrasini, 27-30 Settembre 2006.
305
306
Zaccaroni A.; Gamberoni M.; Mordenti O.; Parmeggiani A.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/41401
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