Tuber magnatum produces the world’s most expensive truffle. This fungus produces very rare ectomycorrhizas which are difficult or even impossible to detect in the field. A ‘‘real-time’’ PCR assay was recently developed to quantify and to track T. magnatum mycelium in soil. Here, this technique was used to investigate bthe spatial distribution of T. magnatum extra-radical mycelium in soil productive patches and its dynamic across seasons. This study was carried out in four different natural T. magnatum truffle grounds located in different Italian regions. During the fruiting seasons, the amount of T. magnatum mycelium was significantly higher around the fruiting points and decreased going farther away from them. Moreover, T. magnatum mycelium inside the productive patches underwent seasonal fluctuations. In early spring, the amount of T. magnatum mycelium was significantly higher than in summer. In summer, probably due to the hot and dry season, T. magnatum mycelium significantly decreased, whereas in autumn it increased again and was concentrated at the putative fruiting points. These results give new insights on T. magnatum ecology and are useful to plan the most appropriate sampling strategy for evaluating the management of a truffle ground.

Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds

IOTTI, MIRCO;LEONARDI, PAMELA;ZAMBONELLI, ALESSANDRA
2014

Abstract

Tuber magnatum produces the world’s most expensive truffle. This fungus produces very rare ectomycorrhizas which are difficult or even impossible to detect in the field. A ‘‘real-time’’ PCR assay was recently developed to quantify and to track T. magnatum mycelium in soil. Here, this technique was used to investigate bthe spatial distribution of T. magnatum extra-radical mycelium in soil productive patches and its dynamic across seasons. This study was carried out in four different natural T. magnatum truffle grounds located in different Italian regions. During the fruiting seasons, the amount of T. magnatum mycelium was significantly higher around the fruiting points and decreased going farther away from them. Moreover, T. magnatum mycelium inside the productive patches underwent seasonal fluctuations. In early spring, the amount of T. magnatum mycelium was significantly higher than in summer. In summer, probably due to the hot and dry season, T. magnatum mycelium significantly decreased, whereas in autumn it increased again and was concentrated at the putative fruiting points. These results give new insights on T. magnatum ecology and are useful to plan the most appropriate sampling strategy for evaluating the management of a truffle ground.
Iotti, Mirco; Leonardi, Marco; Lancellotti, Enrico; Salerni, Elena; Oddis, Marilena; Leonardi, Pamela; Perini, Claudia; Pacioni, Giovanni; Zambonelli, Alessandra
File in questo prodotto:
File Dimensione Formato  
iotti et al 2014journal.pone.0115921.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 845.49 kB
Formato Adobe PDF
845.49 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/406956
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 20
social impact