Iron oxide-containing magnetic nanoparticles (MNPs) have certain advantages over currently used contrast agents for tumor imaging by magnetic resonance imaging (MRI) as they offer the possibility of functionalization with ligands and tracers. Functionalized MNPs alsomay be used for targeted tumor therapy. In the current study nanoparticles (NPs) consisting of recombinant human serum albumin (rHSA) with incorporated hydrophilic (NH4) 2Ce(IV)(NO3)6-γ-Fe2O3 particles (CANmaghemite particles) formedical imaging were produced and characterized. For this purpose CAN maghemite particles were incorporated into an rHSAmatrix to yield rHSA-NPs. The resulting NPs were analyzed by transmission electron microscopy, photon correlation spectroscopy, and atomic absorption. The sizes of the manufactured NP were 170 ± 10 nm, and the zeta-potential was−50±3mV. TheNPs remained stable when stored after lyophilization with sucrose 3% [w/v] as a cryoprotector. They showed pro-inflammatory properties without cell and animal toxicity in vivo and were highly contrasting in MRI. In conclusion, this report introduces novel rHSA NP with favorable properties containing iron oxide for detection by MRI.
Physico-chemical and toxicological characterization of iron-containing albumin nanoparticles as platforms for medical imaging / Ina Rosenberger; Christian Schmithals; Jennifer Vandooren; Silvia Bianchessi; Paolo Milani; Erica Locatelli; Liron L. Israel; Frank Hübner; Michela Matteoli; Jean-Paul Lellouche; Mauro Comes Franchini; Lorena Passoni; Eugenio Scanziani; Ghislain Opdenakker; Albrecht Piiper; Jörg Kreuter. - In: JOURNAL OF CONTROLLED RELEASE. - ISSN 0168-3659. - STAMPA. - 194:(2014), pp. 130-137. [10.1016/j.jconrel.2014.08.017]
Physico-chemical and toxicological characterization of iron-containing albumin nanoparticles as platforms for medical imaging.
LOCATELLI, ERICA;COMES FRANCHINI, MAURO;
2014
Abstract
Iron oxide-containing magnetic nanoparticles (MNPs) have certain advantages over currently used contrast agents for tumor imaging by magnetic resonance imaging (MRI) as they offer the possibility of functionalization with ligands and tracers. Functionalized MNPs alsomay be used for targeted tumor therapy. In the current study nanoparticles (NPs) consisting of recombinant human serum albumin (rHSA) with incorporated hydrophilic (NH4) 2Ce(IV)(NO3)6-γ-Fe2O3 particles (CANmaghemite particles) formedical imaging were produced and characterized. For this purpose CAN maghemite particles were incorporated into an rHSAmatrix to yield rHSA-NPs. The resulting NPs were analyzed by transmission electron microscopy, photon correlation spectroscopy, and atomic absorption. The sizes of the manufactured NP were 170 ± 10 nm, and the zeta-potential was−50±3mV. TheNPs remained stable when stored after lyophilization with sucrose 3% [w/v] as a cryoprotector. They showed pro-inflammatory properties without cell and animal toxicity in vivo and were highly contrasting in MRI. In conclusion, this report introduces novel rHSA NP with favorable properties containing iron oxide for detection by MRI.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
