Volume is one of the most important features for the characterization of a tumour on a macroscopic scale. It is often used to assess the effectiveness of care treatments, thus mak-ing its correct evaluation a crucial issue for patient care. Similarly, volume is a key feature ona microscopic scale. Multicellular cancer spheroids are 3D tumour models widely employedin pre-clinical studies to test the effects of drugs and radiotherapy treatments. Very fewmethods have been proposed to estimate the tumour volume arising from a 2D projectionof multicellular spheroids, and even fewer have been designed to provide a 3D reconstruc-tion of the tumour shape. In this work, we propose Reconstruction and Visualization from aSingle Projection (ReViSP), an automatic method conceived to reconstruct the 3D surface andestimate the volume of single cancer multicellular spheroids, or even of spheroid cultures.As the input parameter ReViSP requires only one 2D projection, which could be a widefieldmicroscope image. We assessed the effectiveness of our method by comparing it with otherapproaches. To this purpose, we used a new strategy that allowed us to achieve accuratevolume measurements based on the analysis of home-made 3D objects, built by mimickingthe spheroid morphology. The results confirmed the effectiveness of our method for both3D reconstruction and volume assessment. ReViSP software is distributed as an open source tool.
Piccinini, F., A., T., C., A., Bevilacqua, A. (2015). Cancer multicellular spheroids: Volume assessment from a single 2D projection. COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, 118(2), 95-106 [10.1016/j.cmpb.2014.12.003].
Cancer multicellular spheroids: Volume assessment from a single 2D projection
PICCININI, FILIPPO;BEVILACQUA, ALESSANDRO
2015
Abstract
Volume is one of the most important features for the characterization of a tumour on a macroscopic scale. It is often used to assess the effectiveness of care treatments, thus mak-ing its correct evaluation a crucial issue for patient care. Similarly, volume is a key feature ona microscopic scale. Multicellular cancer spheroids are 3D tumour models widely employedin pre-clinical studies to test the effects of drugs and radiotherapy treatments. Very fewmethods have been proposed to estimate the tumour volume arising from a 2D projectionof multicellular spheroids, and even fewer have been designed to provide a 3D reconstruc-tion of the tumour shape. In this work, we propose Reconstruction and Visualization from aSingle Projection (ReViSP), an automatic method conceived to reconstruct the 3D surface andestimate the volume of single cancer multicellular spheroids, or even of spheroid cultures.As the input parameter ReViSP requires only one 2D projection, which could be a widefieldmicroscope image. We assessed the effectiveness of our method by comparing it with otherapproaches. To this purpose, we used a new strategy that allowed us to achieve accuratevolume measurements based on the analysis of home-made 3D objects, built by mimickingthe spheroid morphology. The results confirmed the effectiveness of our method for both3D reconstruction and volume assessment. ReViSP software is distributed as an open source tool.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
