The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from 5.08 × 108 to 1.33 × 106 copies/g of total RNA in the hemolymph, 5.096 × 105 to 1.26 × 103 copies/g in the pleopods, 6.85 × 108 to 3.09 × 104 copies/g in muscle and 8.15 × 106 to 3.90 × 103 copies/g in gills. Different viral loads of IMNV were found with greater values in the hemolymph and muscle, followed by the pleopods and gills.
Suzianny Maria Bezerra Cabral da Silva, Ana Cristina de Aguiar Saldanha Pinheiro, Maria Raquel Moura Coimbra (2011). Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry. JOURNAL OF VIROLOGICAL METHODS, 177, 197-201 [10.1016/j.jviromet.2011.08.001].
Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry
DE AGUIAR SALDANHA PINHEIRO, ANA CRISTINA;
2011
Abstract
The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from 5.08 × 108 to 1.33 × 106 copies/g of total RNA in the hemolymph, 5.096 × 105 to 1.26 × 103 copies/g in the pleopods, 6.85 × 108 to 3.09 × 104 copies/g in muscle and 8.15 × 106 to 3.90 × 103 copies/g in gills. Different viral loads of IMNV were found with greater values in the hemolymph and muscle, followed by the pleopods and gills.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.