Direct recordings of electron currents mediated by cytochromes b561 (CYB561) are not available yet, despite the importance of these proteins in a variety of physiological functions, including neurotransmitter synthesis and dietary iron uptake. Here, we used the two-electrode voltage-clamp technique applied to Xenopus oocytes to demonstrate, for the first time, the generation of electron currents by a Drosophila member of the CYB561 superfamily named stromal cell-derived receptor 2 (SDR2). This experimental method, along with the theoretical development of a three-state kinetic model, supports the hypothesis that electron donor/acceptor concentrations and transmembrane voltage mutually control SDR2-mediated electron transport activity in a complex but predictable manner.

How are cytochrome b561 electron currents controlled by membrane voltage and substrate availability? / Picco C.; Scholz-Starke J.; Naso A.; Preger V.; Sparla F.; Trost P.; Carpaneto A.. - In: ANTIOXIDANTS & REDOX SIGNALING. - ISSN 1523-0864. - STAMPA. - 21:3(2014), pp. 384-391. [10.1089/ars.2013.5809]

How are cytochrome b561 electron currents controlled by membrane voltage and substrate availability?

SPARLA, FRANCESCA;TROST, PAOLO BERNARDO;
2014

Abstract

Direct recordings of electron currents mediated by cytochromes b561 (CYB561) are not available yet, despite the importance of these proteins in a variety of physiological functions, including neurotransmitter synthesis and dietary iron uptake. Here, we used the two-electrode voltage-clamp technique applied to Xenopus oocytes to demonstrate, for the first time, the generation of electron currents by a Drosophila member of the CYB561 superfamily named stromal cell-derived receptor 2 (SDR2). This experimental method, along with the theoretical development of a three-state kinetic model, supports the hypothesis that electron donor/acceptor concentrations and transmembrane voltage mutually control SDR2-mediated electron transport activity in a complex but predictable manner.
2014
How are cytochrome b561 electron currents controlled by membrane voltage and substrate availability? / Picco C.; Scholz-Starke J.; Naso A.; Preger V.; Sparla F.; Trost P.; Carpaneto A.. - In: ANTIOXIDANTS & REDOX SIGNALING. - ISSN 1523-0864. - STAMPA. - 21:3(2014), pp. 384-391. [10.1089/ars.2013.5809]
Picco C.; Scholz-Starke J.; Naso A.; Preger V.; Sparla F.; Trost P.; Carpaneto A.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/398075
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 11
social impact