Bio-synthetic scaffolds of interspersed poly(l-lactic acid) (PLLA) and gelatin (GEL) fibers are fabricated by co-electrospinning. Tailored PLLA/GEL compositions are obtained and GEL crosslinking with genipin provides for the maintenance of good fiber morphology. Scaffold tensile mechanical properties are intermediate between those of pure PLLA and GEL and vary as a function of PLLA content. Primary human chondrocytes grown on the scaffolds exhibit good proliferation and increased values of the differentiation parameters, especially for intermediate PLLA/GEL compositions. Mineralization tests enable the deposition of a uniform layer of poorly crystalline apatite onto the scaffolds, suggesting potential applications involving cartilage as well as cartilage-bone interface tissue engineering.

Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition

GIOFFRE', MICHELA;FIORANI, ANDREA;PANZAVOLTA, SILVIA;GUALANDI, CHIARA;FOCARETE, MARIA LETIZIA;BIGI, ADRIANA
2014

Abstract

Bio-synthetic scaffolds of interspersed poly(l-lactic acid) (PLLA) and gelatin (GEL) fibers are fabricated by co-electrospinning. Tailored PLLA/GEL compositions are obtained and GEL crosslinking with genipin provides for the maintenance of good fiber morphology. Scaffold tensile mechanical properties are intermediate between those of pure PLLA and GEL and vary as a function of PLLA content. Primary human chondrocytes grown on the scaffolds exhibit good proliferation and increased values of the differentiation parameters, especially for intermediate PLLA/GEL compositions. Mineralization tests enable the deposition of a uniform layer of poorly crystalline apatite onto the scaffolds, suggesting potential applications involving cartilage as well as cartilage-bone interface tissue engineering.
Paola Torricelli; Michela Gioffrè; Andrea Fiorani; Silvia Panzavolta; Chiara Gualandi; Milena Fini; Maria Letizia Focarete; Adriana Bigi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/396429
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 59
  • ???jsp.display-item.citation.isi??? 60
social impact