Selected cases of canine liposarcomas were classified according with the WHO classification of tumors of domestic animals. When this was not possible the human classification was applied. Cross reactivity of antibodies anti-mdm2 and CDK4 was assessed by western blot analysis. Immunohistochemistry was performed of formalin fixed tissue applying an heat induced antigen retrieval. Cases were considered positive when more than 10% of neoplastic cells had nuclear staining. Forty-seven LP were collected: 19 WDLP, 18 PLP, and 7 MLP. Three cases were consistent with DDLP. Fifteen cases expressed MDM2 and 37 CDK4. MDM2 and CDK4 were respectively expressed in 12/19 and 17/19 cases WDLP, 1/7 and 5/7 MLP, 0/18 and 12/18 PLP, 2/3 and 3/3 DDLP. Taken together WDLPs and DDLPs expressed mdm2 in 14/22 (63.6%) and CDK4 in 20/22 cases (90.9%). These results suggest that WDLP/DDLP may represent a biological entity characterized by MDM2 and CDK4 overexpression, paralleling LP in man, and suggesting the presence of similar gene amplifications.
AVALLONE G., BRUNETTI B., BERNARDINI C., ROCCABIANCA P., FORNI M., LEPRI E., et al. (2015). MDM2 and CDK4 expression in canine liposarcoma. JOURNAL OF COMPARATIVE PATHOLOGY, 152(1), 68-68 [10.1016/j.jcpa.2014.10.106].
MDM2 and CDK4 expression in canine liposarcoma.
AVALLONE, GIANCARLO;BRUNETTI, BARBARA;BERNARDINI, CHIARA;FORNI, MONICA;SARLI, GIUSEPPE
2015
Abstract
Selected cases of canine liposarcomas were classified according with the WHO classification of tumors of domestic animals. When this was not possible the human classification was applied. Cross reactivity of antibodies anti-mdm2 and CDK4 was assessed by western blot analysis. Immunohistochemistry was performed of formalin fixed tissue applying an heat induced antigen retrieval. Cases were considered positive when more than 10% of neoplastic cells had nuclear staining. Forty-seven LP were collected: 19 WDLP, 18 PLP, and 7 MLP. Three cases were consistent with DDLP. Fifteen cases expressed MDM2 and 37 CDK4. MDM2 and CDK4 were respectively expressed in 12/19 and 17/19 cases WDLP, 1/7 and 5/7 MLP, 0/18 and 12/18 PLP, 2/3 and 3/3 DDLP. Taken together WDLPs and DDLPs expressed mdm2 in 14/22 (63.6%) and CDK4 in 20/22 cases (90.9%). These results suggest that WDLP/DDLP may represent a biological entity characterized by MDM2 and CDK4 overexpression, paralleling LP in man, and suggesting the presence of similar gene amplifications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.