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EnglishTwenty-nine canine mammary samples (5 normal mammary glands, 3 atypical ductal hyperplasia, 8 benign and 13 malignant tumors) were tested by immunohistochemistry (IHC) and Western Blot (WB) for E-cadherin. Both methods revealed a reduction of protein expression as the most important finding. A significant reduction of expression was revealed in dysplastic as well in benign and malignant neoplastic samples. The two methods revealed a significant correlation and absence of IHC false negative evaluation when the data of WB were corrected for the amounts of epithelial cells of the sample.
| Titolo: | Determinazione immunoistochimica e western blot della caderina “E” nei tumori mammari della cagna |
| Autore/i: | BRUNETTI, BARBARA; SARLI, GIUSEPPE; BERNARDINI, CHIARA; FORNI, MONICA; BENAZZI, CINZIA |
| Autore/i Unibo: | |
| Anno: | 2004 |
| Rivista: | |
| Pagina iniziale: | 67 |
| Pagina finale: | 67 |
| Abstract: | Twenty-nine canine mammary samples (5 normal mammary glands, 3 atypical ductal hyperplasia, 8 benign and 13 malignant tumors) were tested by immunohistochemistry (IHC) and Western Blot (WB) for E-cadherin. Both methods revealed a reduction of protein expression as the most important finding. A significant reduction of expression was revealed in dysplastic as well in benign and malignant neoplastic samples. The two methods revealed a significant correlation and absence of IHC false negative evaluation when the data of WB were corrected for the amounts of epithelial cells of the sample. |
| Data prodotto definitivo in UGOV: | 11-ott-2005 |
| Appare nelle tipologie: | 4.01 Contributo in Atti di convegno |
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