Twenty-nine canine mammary samples (5 normal mammary glands, 3 atypical ductal hyperplasia, 8 benign and 13 malignant tumors) were tested by immunohistochemistry (IHC) and Western Blot (WB) for E-cadherin. Both methods revealed a reduction of protein expression as the most important finding. A significant reduction of expression was revealed in dysplastic as well in benign and malignant neoplastic samples. The two methods revealed a significant correlation and absence of IHC false negative evaluation when the data of WB were corrected for the amounts of epithelial cells of the sample.
Brunetti, B., Sarli, G., Bernardini, C., Forni, M., Benazzi, C. (2004). Determinazione immunoistochimica e western blot della caderina “E” nei tumori mammari della cagna.
Determinazione immunoistochimica e western blot della caderina “E” nei tumori mammari della cagna
BRUNETTI, BARBARA;SARLI, GIUSEPPE;BERNARDINI, CHIARA;FORNI, MONICA;BENAZZI, CINZIA
2004
Abstract
Twenty-nine canine mammary samples (5 normal mammary glands, 3 atypical ductal hyperplasia, 8 benign and 13 malignant tumors) were tested by immunohistochemistry (IHC) and Western Blot (WB) for E-cadherin. Both methods revealed a reduction of protein expression as the most important finding. A significant reduction of expression was revealed in dysplastic as well in benign and malignant neoplastic samples. The two methods revealed a significant correlation and absence of IHC false negative evaluation when the data of WB were corrected for the amounts of epithelial cells of the sample.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
