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EnglishAim To evaluate the ion-release and the apatite-forming ability (i.e. bioactivity) of a novel, light-curable MTA-containing pulp capping material (TheraCal; Bisco Inc, USA) when immersed in phosphate containing solution. Methodology Sample disks (8 mm dia · 1.6 mm) of TheraCal, Dycal and ProRoot MTA (Dentsply, USA) and Vitrebond (3M; USA) were prepared. TheraCal and Vitrebond samples were light-cured on both surfaces for 20 s using a LED light after application of a transparent polyester strip (Directa Matrix Strips; Directa AB, Sweden). Dycal and ProRoot MTA samples were cured at 37°C, 98% relative humidity for a time 50% longer than the final setting time (ISO6876), i.e. 2 min for Dycal, 117 min for ProRoot MTA. The discs were immersed in 10 mL deionized water and calcium and hydroxyl ion release analyzed using ion-selective electrodes after 3 and 24 h and 3, 7, 14, 28 days at 37°C. For bioactivity evaluation, discs were immersed in 10 mL phosphate-containing solution DPBS (Dulbecco’s Phosphate Buffered Saline) at 37°C. The surface chemistry, morphology and formation of apatite on materials surface after 1, 7, 14, 28 days in DPBS was assessed by ESEM-EDX, micro-Raman and FT-IR techniques. Results TheraCal released the most calcium throughout the test period and alkalinized the surrounding fluid initially to pH 10–11 (3 h–3 day) and subsequently to pH 8 (7–28 day). Amorphous apatite (952 cm)1 Raman band) was detected within the first 24 h, while a more crystalline apatite (960 cm)1 Raman band) was seen at 7 days. A layer of apatite entirely covered the surface of TheraCal after 28 days. Conclusions TheraCal and ProRoot MTA demonstrated the capacity to form apatite after just 24 h in DPBS. Dycal showed limited, and Vitrebond no bioactivity. In this laboratory setting, TheraCal acted as a calcium-releasing material that induced the formation of apatite when in contact with biological fluids. It may have potential to promote new dentine formation and cell proliferation.
| Titolo: | Calcium and hydroxyl ion release and apatite-precipitation triggered by TheraCal, a new light-curable MTA-containing material for pulp-capping | |
| Autore/i: | GANDOLFI, MARIA GIOVANNA; SIBONI, FRANCESCO; TADDEI, PAOLA; B. Suh; PRATI, CARLO | |
| Autore/i Unibo: | ||
| Anno: | 2011 | |
| Rivista: | ||
| Abstract: | Aim To evaluate the ion-release and the apatite-forming ability (i.e. bioactivity) of a novel, light-curable MTA-containing pulp capping material (TheraCal; Bisco Inc, USA) when immersed in phosphate containing solution. Methodology Sample disks (8 mm dia · 1.6 mm) of TheraCal, Dycal and ProRoot MTA (Dentsply, USA) and Vitrebond (3M; USA) were prepared. TheraCal and Vitrebond samples were light-cured on both surfaces for 20 s using a LED light after application of a transparent polyester strip (Directa Matrix Strips; Directa AB, Sweden). Dycal and ProRoot MTA samples were cured at 37°C, 98% relative humidity for a time 50% longer than the final setting time (ISO6876), i.e. 2 min for Dycal, 117 min for ProRoot MTA. The discs were immersed in 10 mL deionized water and calcium and hydroxyl ion release analyzed using ion-selective electrodes after 3 and 24 h and 3, 7, 14, 28 days at 37°C. For bioactivity evaluation, discs were immersed in 10 mL phosphate-containing solution DPBS (Dulbecco’s Phosphate Buffered Saline) at 37°C. The surface chemistry, morphology and formation of apatite on materials surface after 1, 7, 14, 28 days in DPBS was assessed by ESEM-EDX, micro-Raman and FT-IR techniques. Results TheraCal released the most calcium throughout the test period and alkalinized the surrounding fluid initially to pH 10–11 (3 h–3 day) and subsequently to pH 8 (7–28 day). Amorphous apatite (952 cm)1 Raman band) was detected within the first 24 h, while a more crystalline apatite (960 cm)1 Raman band) was seen at 7 days. A layer of apatite entirely covered the surface of TheraCal after 28 days. Conclusions TheraCal and ProRoot MTA demonstrated the capacity to form apatite after just 24 h in DPBS. Dycal showed limited, and Vitrebond no bioactivity. In this laboratory setting, TheraCal acted as a calcium-releasing material that induced the formation of apatite when in contact with biological fluids. It may have potential to promote new dentine formation and cell proliferation. | |
| Data prodotto definitivo in UGOV: | 19-nov-2014 | |
| Appare nelle tipologie: | 1.06 Abstract in rivista |
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