Data on proteinuria in giraffes are lacking, therefore the aim of our research was to study urine proteome with sensitive analytical methods to obtain important information regarding not only the kidney function but also the general health status. Fifty urine samples were collected during 2013 and 2014, through non-invasive methods from 21 specimens of Giraffa camelopardalis. Urinary total proteins (UTP), creatinine and urine protein to creatinine ratio (UP/C) were measured by automated methods routinely used in small animal practice. Urinary proteins were separated by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and gels were stained with silver nitrate. Mean UTP±SD was 16.1±8.8 mg/dL (range 2.4-30 mg/dL), while mean UP/C was 0.08±0.03 (range 0.04-0.2); these values can be considered representative of healthy animals. SDS-PAGE yielded an overall view of the urine protein profile allowing the separation of 6±2 protein bands. Electropherograms showed few protein bands as compared to dogs, cats (Ferlizza et al., 2012) and big felids (Di Cerbo et al., 2013). The most represented band had an apparent molecular weight (MW) of 67-69 kDa and could be identified as putative albumin. Between 65 and 14 kDa only few low abundance protein bands were present, while more intense bands had a MW lower than 14 kDa. This is the first study reporting UTP and UPC ranges in giraffes. 1-D-SDS-PAGE yielded an overall view of the protein profile and resulted in a useful diagnostic tool that could help clinicians in qualitative evaluation of proteinuria. References Di Cerbo P., Ferrari G., Isani G., Ferlizza E., Friedrich K.G., 2013. Urine proteome in big cats (Panthera leo, Panthera tigris, Panthera pardus). In: proceedings of the International Conference on Diseases of Zoo and Wild Animals, Vienna, A. Ferlizza E., Armuzzi E., Buono A., Carpenè E., Andreani G., Dondi F., Isani G., 2012. Urine proteome from healthy dogs, cats, horses and cows. In: proceedings of the European Society of Veterinary Clinical Pathology Conference, Ljubljana, SLO.
Urine proteome evaluation in Giraffes (Giraffa camelopardalis) from Italian Zoos / FERLIZZA E; FASOLI S; DONDI F; SCHNEIDER R; SANDRI C; DI GIROLAMO N; ISANI G. - STAMPA. - (2015), pp. 1-1. (Intervento presentato al convegno ICARE 2015 tenutosi a Paris nel 18-23 April 2015).
Urine proteome evaluation in Giraffes (Giraffa camelopardalis) from Italian Zoos
FERLIZZA, ENEA;FASOLI S;DONDI, FRANCESCO;DI GIROLAMO, NICOLA;ISANI, GLORIA
2015
Abstract
Data on proteinuria in giraffes are lacking, therefore the aim of our research was to study urine proteome with sensitive analytical methods to obtain important information regarding not only the kidney function but also the general health status. Fifty urine samples were collected during 2013 and 2014, through non-invasive methods from 21 specimens of Giraffa camelopardalis. Urinary total proteins (UTP), creatinine and urine protein to creatinine ratio (UP/C) were measured by automated methods routinely used in small animal practice. Urinary proteins were separated by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and gels were stained with silver nitrate. Mean UTP±SD was 16.1±8.8 mg/dL (range 2.4-30 mg/dL), while mean UP/C was 0.08±0.03 (range 0.04-0.2); these values can be considered representative of healthy animals. SDS-PAGE yielded an overall view of the urine protein profile allowing the separation of 6±2 protein bands. Electropherograms showed few protein bands as compared to dogs, cats (Ferlizza et al., 2012) and big felids (Di Cerbo et al., 2013). The most represented band had an apparent molecular weight (MW) of 67-69 kDa and could be identified as putative albumin. Between 65 and 14 kDa only few low abundance protein bands were present, while more intense bands had a MW lower than 14 kDa. This is the first study reporting UTP and UPC ranges in giraffes. 1-D-SDS-PAGE yielded an overall view of the protein profile and resulted in a useful diagnostic tool that could help clinicians in qualitative evaluation of proteinuria. References Di Cerbo P., Ferrari G., Isani G., Ferlizza E., Friedrich K.G., 2013. Urine proteome in big cats (Panthera leo, Panthera tigris, Panthera pardus). In: proceedings of the International Conference on Diseases of Zoo and Wild Animals, Vienna, A. Ferlizza E., Armuzzi E., Buono A., Carpenè E., Andreani G., Dondi F., Isani G., 2012. Urine proteome from healthy dogs, cats, horses and cows. In: proceedings of the European Society of Veterinary Clinical Pathology Conference, Ljubljana, SLO.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
