Intrapartum antibiotic prophylaxis (IAP) is the most effective strategy to prevent early-onset Group B Streptococcal (GBS) sepsis. A possible role of IAP on neonatal microbiota is assumed. We aimed to evaluate the effect of IAP on the bacterial colonisation of neonatal gut at 7 and 30 days of life (DOL). Term newborns, vaginally delivered, were recruited at 2 DOL and allocated into four groups. Group 1: infants exclusively breastfed, not exposed to IAP. Group 2: infants partially breastfed (receiving at least 50% of own mother’s milk), not exposed to IAP. Group 3: infants exclusively breastfed, exposed to IAP. Group 4: infants partially breastfed, exposed to IAP. Faecal samples from the enrolled infants were collected at 7 and 30 DOL. The count of Bifidobacteriumspp., assessed by real-time PCR, was compared between the four groups. Fifty-five newborns were recruited: 25 in Group 1, 7 in Group 2, 17 in Group 3, 6 in Group 4. On day 7, IAP-exposed newborns showed a significantly lower count ofBifidobacterium spp. (p <0.05). Among infants not exposed to IAP,Bifidobacterium spp. count was significantly higher in Group 1 compared to Group 2. On day 30, a significant increase in Bifidobacterium spp. count (p <0.05) compared to day 7 was observed in all groups. Early neonatal microbiota is significantly affected by IAP, resulting in a reduced Bifidobacteria colonisation. Breastfeeding promotes the development of bifidogenic flora and possibly contributes to increase Bifidobacteriumspp. count in IAPexposed newborns ad 30 DOL.

DEVELOPMENT OF GUT MICROBIOTA: EFFECT OF INTRAPARTUM ANTIBIOTIC PROPHYLAXIS IN BREASTFED TERM NEWBORNS / G Tonti; S Martini; L Corvaglia; I Aloisio; G Mazzola; D Di Gioia; G Faldella. - In: ARCHIVES OF DISEASE IN CHILDHOOD. - ISSN 0003-9888. - ELETTRONICO. - 99:(2014), pp. 179-179. [10.1136/archdischild-2014-307384.484]

DEVELOPMENT OF GUT MICROBIOTA: EFFECT OF INTRAPARTUM ANTIBIOTIC PROPHYLAXIS IN BREASTFED TERM NEWBORNS

MARTINI, SILVIA;CORVAGLIA, LUIGI TOMMASO;ALOISIO, IRENE;MAZZOLA, GIUSEPPE;DI GIOIA, DIANA;FALDELLA, GIACOMO
2014

Abstract

Intrapartum antibiotic prophylaxis (IAP) is the most effective strategy to prevent early-onset Group B Streptococcal (GBS) sepsis. A possible role of IAP on neonatal microbiota is assumed. We aimed to evaluate the effect of IAP on the bacterial colonisation of neonatal gut at 7 and 30 days of life (DOL). Term newborns, vaginally delivered, were recruited at 2 DOL and allocated into four groups. Group 1: infants exclusively breastfed, not exposed to IAP. Group 2: infants partially breastfed (receiving at least 50% of own mother’s milk), not exposed to IAP. Group 3: infants exclusively breastfed, exposed to IAP. Group 4: infants partially breastfed, exposed to IAP. Faecal samples from the enrolled infants were collected at 7 and 30 DOL. The count of Bifidobacteriumspp., assessed by real-time PCR, was compared between the four groups. Fifty-five newborns were recruited: 25 in Group 1, 7 in Group 2, 17 in Group 3, 6 in Group 4. On day 7, IAP-exposed newborns showed a significantly lower count ofBifidobacterium spp. (p <0.05). Among infants not exposed to IAP,Bifidobacterium spp. count was significantly higher in Group 1 compared to Group 2. On day 30, a significant increase in Bifidobacterium spp. count (p <0.05) compared to day 7 was observed in all groups. Early neonatal microbiota is significantly affected by IAP, resulting in a reduced Bifidobacteria colonisation. Breastfeeding promotes the development of bifidogenic flora and possibly contributes to increase Bifidobacteriumspp. count in IAPexposed newborns ad 30 DOL.
2014
DEVELOPMENT OF GUT MICROBIOTA: EFFECT OF INTRAPARTUM ANTIBIOTIC PROPHYLAXIS IN BREASTFED TERM NEWBORNS / G Tonti; S Martini; L Corvaglia; I Aloisio; G Mazzola; D Di Gioia; G Faldella. - In: ARCHIVES OF DISEASE IN CHILDHOOD. - ISSN 0003-9888. - ELETTRONICO. - 99:(2014), pp. 179-179. [10.1136/archdischild-2014-307384.484]
G Tonti; S Martini; L Corvaglia; I Aloisio; G Mazzola; D Di Gioia; G Faldella
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/371515
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