A precise and feasible high-performance liquid chromatographic (HPLC) method has been developed for the analysis of amphetamine (AMPH), metamphetamine (MAMPH) and methylendioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 x 4.6 mm, 5 µm) column maintained at 30°C lasts about 17 minutes, using a mobile phase composed of acetonitrile (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values > 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of amphetamine users.
Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure / F. Bugamelli; R. Mandrioli; A. Cavallini; C. Baccini; M. Conti; M.A. Raggi. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9306. - STAMPA. - 29:(2006), pp. 2322-2329. [10.1002/jssc.200600139]
Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid-phase extraction procedure
BUGAMELLI, FRANCESCA;MANDRIOLI, ROBERTO;RAGGI, MARIA AUGUSTA
2006
Abstract
A precise and feasible high-performance liquid chromatographic (HPLC) method has been developed for the analysis of amphetamine (AMPH), metamphetamine (MAMPH) and methylendioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 x 4.6 mm, 5 µm) column maintained at 30°C lasts about 17 minutes, using a mobile phase composed of acetonitrile (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values > 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of amphetamine users.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
