Bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) still results in serious yield losses in Italy and, despite the studies conducted hitherto to deal with this devastating disease, some epidemiological aspects are still to be clarified. In particular, the available information on life-cycle of Psa is still incomplete on the latency period of Psa within the susceptible host plant. To elucidate this dangerous endophytic latent phase, the survival of a virulent Psa gfp-expressing/Rif-resistant strain (Psa::gfp) within asymptomatic Actinidia spp. plants was studied. The results were obtained both in adult plants of Actinidia chinensis cv. Hort16A four years after inoculation with Psa::gfp at low inoculum dose, and in Actinidia deliciosa cv. Hayward plants obtained from micropropagated shoots similarly inoculated more than three years before. Both types of plant were in toto analyzed to re-isolate Psa::gfp on selective media, and to check its ability to induce disease symptoms in host plants and hypersensitivity reaction in tobacco plants. PCR analysis was carried out to confirm the identity of the re-isolate Psa::gfp. On the fourth year following inoculation, the data confirmed that Psa can colonize plants for a long time staying latent at very low concentrations. In the pathosystem Actinidia-Psa the pathogen appears to have achieved a high degree of pathoadaptation which is witnessed in a pluriannual latency period. The implications of what reported are especially important for its impact on the nursery industry, and control strategies in kiwifruit orchards to prevent the spread of the pathogen present in asymptomatic plants.

HOST-PATHOGEN INTERACTION IN ACTINIDIA-BACTERIAL CANKER PATHOSYSTEM: INVESTIGATION ON THE LATENCY PERIOD IN ASYMPTOMATIC PLANTS (vol 96, pg 38, 2014)

MINARDI, PAOLA;ARDIZZI, STEFANO;LUCCHESE, CARLA;BERTACCINI, ASSUNTA
2016

Abstract

Bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) still results in serious yield losses in Italy and, despite the studies conducted hitherto to deal with this devastating disease, some epidemiological aspects are still to be clarified. In particular, the available information on life-cycle of Psa is still incomplete on the latency period of Psa within the susceptible host plant. To elucidate this dangerous endophytic latent phase, the survival of a virulent Psa gfp-expressing/Rif-resistant strain (Psa::gfp) within asymptomatic Actinidia spp. plants was studied. The results were obtained both in adult plants of Actinidia chinensis cv. Hort16A four years after inoculation with Psa::gfp at low inoculum dose, and in Actinidia deliciosa cv. Hayward plants obtained from micropropagated shoots similarly inoculated more than three years before. Both types of plant were in toto analyzed to re-isolate Psa::gfp on selective media, and to check its ability to induce disease symptoms in host plants and hypersensitivity reaction in tobacco plants. PCR analysis was carried out to confirm the identity of the re-isolate Psa::gfp. On the fourth year following inoculation, the data confirmed that Psa can colonize plants for a long time staying latent at very low concentrations. In the pathosystem Actinidia-Psa the pathogen appears to have achieved a high degree of pathoadaptation which is witnessed in a pluriannual latency period. The implications of what reported are especially important for its impact on the nursery industry, and control strategies in kiwifruit orchards to prevent the spread of the pathogen present in asymptomatic plants.
Minardi P.; Ardizzi S.; Lucchese C.; Bertaccini A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/345316
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