The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50: 0.052 and 0.075 μM, respectively) than CoQ3 (IC50: 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation.
Rauchova H., Vrbacky M., Bergamini C., Fato R., Lenaz G., Houstek J., et al. (2006). Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 339, 362-366 [10.1016/j.bbrc.2005.11.035].
Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone
BERGAMINI, CHRISTIAN;FATO, ROMANA;LENAZ, GIORGIO;
2006
Abstract
The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50: 0.052 and 0.075 μM, respectively) than CoQ3 (IC50: 45.8 μM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
