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EnglishThe use of anisoin as pre-chromatographic reagent for LC analysis of guanidino compounds is proposed. The reagent reacts (5 min at 100°C) with guanidino function and the resulting adducts can be chromatographed under reversed-phase conditions. A fluorescence detector (lex = 325 nm; lem = 435 nm) was used to detect guanidino adducts. The derivatization and chromatographic conditions were optimised by a series of experiments. Application to the determination of arginine and creatine in pharmaceuticals and arginine, guanidine, methylguanidine, guanidinosuccinic acid, b-guanidinopropionic acid, g-guanidinobutyric acid, guanidinoacetic acid and homoarginine in human urine is described. Quantitation limits ranged from 6 to 30 fmol, except for creatine (510 fmol).
| Titolo: | Anisoin: A useful pre-chromatographic derivatization fluorogenic reagent for LC analysis of guanidino compounds | |
| Autore/i: | GATTI, RITA; GIOIA, MARIA GRAZIA | |
| Autore/i Unibo: | ||
| Anno: | 2006 | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.jpba.2005.12.035 | |
| Abstract: | The use of anisoin as pre-chromatographic reagent for LC analysis of guanidino compounds is proposed. The reagent reacts (5 min at 100°C) with guanidino function and the resulting adducts can be chromatographed under reversed-phase conditions. A fluorescence detector (lex = 325 nm; lem = 435 nm) was used to detect guanidino adducts. The derivatization and chromatographic conditions were optimised by a series of experiments. Application to the determination of arginine and creatine in pharmaceuticals and arginine, guanidine, methylguanidine, guanidinosuccinic acid, b-guanidinopropionic acid, g-guanidinobutyric acid, guanidinoacetic acid and homoarginine in human urine is described. Quantitation limits ranged from 6 to 30 fmol, except for creatine (510 fmol). | |
| Data prodotto definitivo in UGOV: | 2006-10-17 14:56:51 | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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