Turnera ulmifolia, the yellow alder, is a widely distributed species in Brazil where, besides ornamental, it is used for its medicinal properties. Plants of T. ulmifolia exhibiting witches’ broom growths and yellowing, that are symptoms typically associated with phytoplasma presence, have been observed in the location of Penedo, state of Rio de Janeiro. Symptomatic samples from T. ulmifolia were collected and after total nucleic acid extraction, universal primer pairs were used to prime amplification of phytoplasma 16S rDNA sequences, spacer region and beginning of the 23S rDNA. Expected length amplicons of 1.5 kb (F1/B6 primers) and 1.2 kb (R16F2/R2 primers) were obtained from all symptomatic samples tested after nested PCR on P1/P7 amplicons. RFLP analyses were carried out with Tru1I on F1/B6 and R16F2/R2 amplicons; and with TaqI, and AluI on R16F2/R2 amplicons. RFLP patterns were compared with those of phytoplasma reference strains on same size amplicons and allow to identify the detected phytoplasmas as belonging to ribosomal group 16SrXIII. Additional amplifications for molecular characterization of T. ulmifolia phytoplasmas, with primers rp(I)F1/rp(I)R1A amplifying rplV (rpl22) and rpsC (rps3) genes were then performed. The amplification of this gene resulted in the expected 1.2 kb amplicons and the RFLP profile obtained after Tru1I digestion was clearly different from any of those available in the literature for the same gene, indicating that this phytoplasma may represent a new strain in 16SrXIII group.
Montano H.G., N. Contaldo, J.P. Pimentel, J.O. Cunha Jr, S. Paltrinieri, A. Bertaccini (2014). Multigene characterization of phytoplasmas infecting Turnera ulmifolia in Brazil. Blumenau : FURB.
Multigene characterization of phytoplasmas infecting Turnera ulmifolia in Brazil
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2014
Abstract
Turnera ulmifolia, the yellow alder, is a widely distributed species in Brazil where, besides ornamental, it is used for its medicinal properties. Plants of T. ulmifolia exhibiting witches’ broom growths and yellowing, that are symptoms typically associated with phytoplasma presence, have been observed in the location of Penedo, state of Rio de Janeiro. Symptomatic samples from T. ulmifolia were collected and after total nucleic acid extraction, universal primer pairs were used to prime amplification of phytoplasma 16S rDNA sequences, spacer region and beginning of the 23S rDNA. Expected length amplicons of 1.5 kb (F1/B6 primers) and 1.2 kb (R16F2/R2 primers) were obtained from all symptomatic samples tested after nested PCR on P1/P7 amplicons. RFLP analyses were carried out with Tru1I on F1/B6 and R16F2/R2 amplicons; and with TaqI, and AluI on R16F2/R2 amplicons. RFLP patterns were compared with those of phytoplasma reference strains on same size amplicons and allow to identify the detected phytoplasmas as belonging to ribosomal group 16SrXIII. Additional amplifications for molecular characterization of T. ulmifolia phytoplasmas, with primers rp(I)F1/rp(I)R1A amplifying rplV (rpl22) and rpsC (rps3) genes were then performed. The amplification of this gene resulted in the expected 1.2 kb amplicons and the RFLP profile obtained after Tru1I digestion was clearly different from any of those available in the literature for the same gene, indicating that this phytoplasma may represent a new strain in 16SrXIII group.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
