Quantitative and qualitative parameters of Exorista larvarum (L.) (Diptera: Tachinidae) reared on two insect material-free artificial media and in the factitious host Galleria mellonella L were compared. Significantly higher puparial yields and weights were obtained in both a milk-based and a veal homogenate-based medium than in the factitious host. Longevity and parasitization rates were not different between the in vitro- and the in vivo-reared flies. Despite the larger puparial weight of the veal medium-reared E. larvarum females, the number of eggs laid by these females on host larvae was not found to be higher than that of the females reared under the other two feeding conditions. Moreover, in a complementary experiment, despite the homogenous puparial weights of milk medium- and host-reared females, the former oviposited less eggs . Hence, puparial weight alone is not a reliable quality parameter for E. larvarum reared on artificial media. Lower amino acid content with a deficiency in aromatic amino acids and an excess in proline was found for in vitro 3rd instar parasitoid larvae reared on both media compared to the in vivo reared ones. A correlation between the lower amino acid content of medium-reared larvae and the lower number of eggs laid by the females obtained, compared to controls, may be hypothesised.

Biological and biochemical differences between in vitro- and in vivo-reared Exorista larvarum

DINDO, MARIA LUISA;BARONIO, PIERO
2006

Abstract

Quantitative and qualitative parameters of Exorista larvarum (L.) (Diptera: Tachinidae) reared on two insect material-free artificial media and in the factitious host Galleria mellonella L were compared. Significantly higher puparial yields and weights were obtained in both a milk-based and a veal homogenate-based medium than in the factitious host. Longevity and parasitization rates were not different between the in vitro- and the in vivo-reared flies. Despite the larger puparial weight of the veal medium-reared E. larvarum females, the number of eggs laid by these females on host larvae was not found to be higher than that of the females reared under the other two feeding conditions. Moreover, in a complementary experiment, despite the homogenous puparial weights of milk medium- and host-reared females, the former oviposited less eggs . Hence, puparial weight alone is not a reliable quality parameter for E. larvarum reared on artificial media. Lower amino acid content with a deficiency in aromatic amino acids and an excess in proline was found for in vitro 3rd instar parasitoid larvae reared on both media compared to the in vivo reared ones. A correlation between the lower amino acid content of medium-reared larvae and the lower number of eggs laid by the females obtained, compared to controls, may be hypothesised.
Dindo M.L.; Grenier S.; Sighinolfi L.; Baronio P.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/30362
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