Thyroid carcinoma is the most common endocrine malignancy, and papillary thyroid carcinoma represents the most common thyroid cancer. Papillary thyroid carcinomas that invade locally or metastasize are associated with a poor prognosis. We found that, during epithelialemesenchymal transition (EMT) induced by transforming growth factor-b1 (TGF-b1), papillary thyroid carcinoma cells acquired Q5 increased cancer stem cell-like features and the transcription factor paired-related homeobox protein 1 (PRX-1; alias PRRX1), a newly identified EMT inducer, was markedly up-regulated. miR-146b-5p was also transiently up-regulated during EMT, and in siRNA experiments miR-146b-5p had an inhibitory role on cell proliferation and invasion during TGF-b1einduced EMT. We conclude that papillary thyroid carcinoma tumor cells exhibit increased cancer stem cell-like features during TGF-b1einduced EMT, that miR-146b-5p has a role in cell proliferation and invasion, and that PRRX1 plays an important role in papillary thyroid carcinoma EMT and disease progression
Hardin H, Guo Z, Shan W, Montemayor-Garcia C, Asioli S, Yu XM, et al. (2014). The Roles of the Epithelial-Mesenchymal Transition Marker PRRX1 and MicroRNA 146b-5p in Papillary Thyroid Carcinoma Progression. THE AMERICAN JOURNAL OF PATHOLOGY, 8, 2342-2354 [10.1016/j.ajpath.2014.04.011].
The Roles of the Epithelial-Mesenchymal Transition Marker PRRX1 and MicroRNA 146b-5p in Papillary Thyroid Carcinoma Progression.
ASIOLI, SOFIA;
2014
Abstract
Thyroid carcinoma is the most common endocrine malignancy, and papillary thyroid carcinoma represents the most common thyroid cancer. Papillary thyroid carcinomas that invade locally or metastasize are associated with a poor prognosis. We found that, during epithelialemesenchymal transition (EMT) induced by transforming growth factor-b1 (TGF-b1), papillary thyroid carcinoma cells acquired Q5 increased cancer stem cell-like features and the transcription factor paired-related homeobox protein 1 (PRX-1; alias PRRX1), a newly identified EMT inducer, was markedly up-regulated. miR-146b-5p was also transiently up-regulated during EMT, and in siRNA experiments miR-146b-5p had an inhibitory role on cell proliferation and invasion during TGF-b1einduced EMT. We conclude that papillary thyroid carcinoma tumor cells exhibit increased cancer stem cell-like features during TGF-b1einduced EMT, that miR-146b-5p has a role in cell proliferation and invasion, and that PRRX1 plays an important role in papillary thyroid carcinoma EMT and disease progressionI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
