In recent years, the evaluation of lymphoproliferative malignancies by molecular assays has become increasingly popular in human medicine. Since microscopic techniques are often unable to recognise a residual neoplastic population whenever they account for less than 1% of the total cells, molecular tools are mainly used for the assessment of the MRD ( Campana et al., 1995; Rezuke et al., 1997). Many different molecular markers have been evaluated in order to increase the sensitivity of the diagnostic tools; among these, the clonal rearrangements of immunoglobulin (VDJ segments) and TCR receptor (TRCγ V and J segments) genes, have been investigated. A PCR assay for the identification of BCR and TCR clonal rearrangements has also recently validated in veterinary medicine; this assay was used in association with cytology and/or histology for diagnosing lymphoproliferative diseases in dogs (Burnett et al., 2003; Dreitz et al., 1999; Vernau et al., 1999). The aim of this work was to evaluate the usefulness of this PCR assay in identification of MRD in lymphoma-affected dogs, in order to modulate therapeutic protocols to obtain longer remission times.

PCR assessment of minimal residual disease in 8 lymphoma-affected dogs

CALZOLARI, CLAUDIA;GENTILINI, FABIO;AGNOLI, CHIARA;ZANNONI, AUGUSTA;PELI, ANGELO;CINOTTI, STEFANO;FAMIGLI BERGAMINI, PAOLO
2006

Abstract

In recent years, the evaluation of lymphoproliferative malignancies by molecular assays has become increasingly popular in human medicine. Since microscopic techniques are often unable to recognise a residual neoplastic population whenever they account for less than 1% of the total cells, molecular tools are mainly used for the assessment of the MRD ( Campana et al., 1995; Rezuke et al., 1997). Many different molecular markers have been evaluated in order to increase the sensitivity of the diagnostic tools; among these, the clonal rearrangements of immunoglobulin (VDJ segments) and TCR receptor (TRCγ V and J segments) genes, have been investigated. A PCR assay for the identification of BCR and TCR clonal rearrangements has also recently validated in veterinary medicine; this assay was used in association with cytology and/or histology for diagnosing lymphoproliferative diseases in dogs (Burnett et al., 2003; Dreitz et al., 1999; Vernau et al., 1999). The aim of this work was to evaluate the usefulness of this PCR assay in identification of MRD in lymphoma-affected dogs, in order to modulate therapeutic protocols to obtain longer remission times.
Calzolari C.; Gentilini F.; Agnoli C.; Zannoni A.; Peli A.; Cinotti S.; Famigli Bergamini P.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/27718
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