The interaction between lipid vesicles and NGF(1-14) peptide, mimicking nerve growth factor, was addressed to fabricate peptide-associated supported lipid bilayers (SLBs). According to a model of predominant electrostatic interactions, zwitterionic and anionic lipid vesicles were used to optimize the peptide association with the lipid membranes. Both planar silica and core-shell nanoparticles (NPs) were used as polar hydrophilic substrates to form the SLBs functionalized with the NGF peptide. The hybrid biointerface was scrutinized by a multitechnique approach with QCM-D, FRAP and fluorescence spectroscopy in terms of self-assembling kinetics, lipid lateral diffusion, and energy transfer processes in the SLB-wrapped silica NPs dye-doped in the core. The response of neuronal cells to the NGF(1-14)-SLBs highlighted their promising application as a drug delivery nanoplatform for ageing-related diseases.

Alessio Travaglia, Cristina Satriano, Maria Laura Giuffrida, Diego La Mendola, Enrico Rampazzo, Luca Prodi, et al. (2013). Electrostatically driven interaction of silica-supported lipid bilayer nanoplatforms and a nerve growth factor-mimicking peptide. SOFT MATTER, 9(18), 4648-4654 [10.1039/c3sm50628b].

Electrostatically driven interaction of silica-supported lipid bilayer nanoplatforms and a nerve growth factor-mimicking peptide

RAMPAZZO, ENRICO;PRODI, LUCA;
2013

Abstract

The interaction between lipid vesicles and NGF(1-14) peptide, mimicking nerve growth factor, was addressed to fabricate peptide-associated supported lipid bilayers (SLBs). According to a model of predominant electrostatic interactions, zwitterionic and anionic lipid vesicles were used to optimize the peptide association with the lipid membranes. Both planar silica and core-shell nanoparticles (NPs) were used as polar hydrophilic substrates to form the SLBs functionalized with the NGF peptide. The hybrid biointerface was scrutinized by a multitechnique approach with QCM-D, FRAP and fluorescence spectroscopy in terms of self-assembling kinetics, lipid lateral diffusion, and energy transfer processes in the SLB-wrapped silica NPs dye-doped in the core. The response of neuronal cells to the NGF(1-14)-SLBs highlighted their promising application as a drug delivery nanoplatform for ageing-related diseases.
2013
Alessio Travaglia, Cristina Satriano, Maria Laura Giuffrida, Diego La Mendola, Enrico Rampazzo, Luca Prodi, et al. (2013). Electrostatically driven interaction of silica-supported lipid bilayer nanoplatforms and a nerve growth factor-mimicking peptide. SOFT MATTER, 9(18), 4648-4654 [10.1039/c3sm50628b].
Alessio Travaglia;Cristina Satriano;Maria Laura Giuffrida;Diego La Mendola;Enrico Rampazzo;Luca Prodi;Enrico Rizzarelli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/267898
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