The interaction between lipid vesicles and NGF(1-14) peptide, mimicking nerve growth factor, was addressed to fabricate peptide-associated supported lipid bilayers (SLBs). According to a model of predominant electrostatic interactions, zwitterionic and anionic lipid vesicles were used to optimize the peptide association with the lipid membranes. Both planar silica and core-shell nanoparticles (NPs) were used as polar hydrophilic substrates to form the SLBs functionalized with the NGF peptide. The hybrid biointerface was scrutinized by a multitechnique approach with QCM-D, FRAP and fluorescence spectroscopy in terms of self-assembling kinetics, lipid lateral diffusion, and energy transfer processes in the SLB-wrapped silica NPs dye-doped in the core. The response of neuronal cells to the NGF(1-14)-SLBs highlighted their promising application as a drug delivery nanoplatform for ageing-related diseases.
Electrostatically driven interaction of silica-supported lipid bilayer nanoplatforms and a nerve growth factor-mimicking peptide / Alessio Travaglia;Cristina Satriano;Maria Laura Giuffrida;Diego La Mendola;Enrico Rampazzo;Luca Prodi;Enrico Rizzarelli. - In: SOFT MATTER. - ISSN 1744-683X. - STAMPA. - 9:18(2013), pp. 4648-4654. [10.1039/c3sm50628b]
Electrostatically driven interaction of silica-supported lipid bilayer nanoplatforms and a nerve growth factor-mimicking peptide
RAMPAZZO, ENRICO;PRODI, LUCA;
2013
Abstract
The interaction between lipid vesicles and NGF(1-14) peptide, mimicking nerve growth factor, was addressed to fabricate peptide-associated supported lipid bilayers (SLBs). According to a model of predominant electrostatic interactions, zwitterionic and anionic lipid vesicles were used to optimize the peptide association with the lipid membranes. Both planar silica and core-shell nanoparticles (NPs) were used as polar hydrophilic substrates to form the SLBs functionalized with the NGF peptide. The hybrid biointerface was scrutinized by a multitechnique approach with QCM-D, FRAP and fluorescence spectroscopy in terms of self-assembling kinetics, lipid lateral diffusion, and energy transfer processes in the SLB-wrapped silica NPs dye-doped in the core. The response of neuronal cells to the NGF(1-14)-SLBs highlighted their promising application as a drug delivery nanoplatform for ageing-related diseases.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
