A new turn-on Cu2+ fluorescent sensor (CST) having a trehalose moiety, which confers a relatively large solubility in water, has been synthesized. The chemosensor is therefore suitable for studies in aqueous solution. Full potentiometric and UV-vis characterization evidence that at physiological pH CST forms with Cu2+ a species with a 1 : 1 stoichiometry allowing for a straightforward correlation between CST response and copper(II) concentration. The presence of the trehalose unit does not negatively affect the selectivity of CST for Cu2+ over a series of metal ions of interest as proven by fluorescence measurements. The novel chemosensor, tested in differentiated neuroblastoma SH-SY5Y cells, is able to detect Cu2+ in the extracellular region, as well as to track copper transfer processes upon cell stimulation induced by cellular depolarization.
Giuseppa Ida Grasso, Salvatore Gentile, Maria Laura Giuffrida, Cristina Satriano, Carmelo Sgarlata, Massimo Sgarzi, et al. (2013). Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor. RSC ADVANCES, 3(46), 24288-24297 [10.1039/c3ra43988g].
Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor
SGARZI, MASSIMO;PRODI, LUCA
2013
Abstract
A new turn-on Cu2+ fluorescent sensor (CST) having a trehalose moiety, which confers a relatively large solubility in water, has been synthesized. The chemosensor is therefore suitable for studies in aqueous solution. Full potentiometric and UV-vis characterization evidence that at physiological pH CST forms with Cu2+ a species with a 1 : 1 stoichiometry allowing for a straightforward correlation between CST response and copper(II) concentration. The presence of the trehalose unit does not negatively affect the selectivity of CST for Cu2+ over a series of metal ions of interest as proven by fluorescence measurements. The novel chemosensor, tested in differentiated neuroblastoma SH-SY5Y cells, is able to detect Cu2+ in the extracellular region, as well as to track copper transfer processes upon cell stimulation induced by cellular depolarization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
