Amyloid beta 25-35 [A beta (25-35)], as a peptide model for full-Length A beta in structural and functional investigations, has been chosen for aggregation studies. The complexity of the A beta (25-35) aggregation process required a multi-methodological analytical approach to obtain reliable and reproducible results. Here, we describe the results obtained by the use of mass spectrometry (MS) for the structural characterization of the self-assembly species during the aggregation process and for the definition of the self-assembly kinetics and myricetin inhibition patterns, comparing the results with those obtained by using the well-established spectroscopic method based on thioflavin T fluorescence. Flow injection electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) was applied to monitor the disappearance of the monomer specie in the first steps, whereas matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-ToF-MS) was used to follow monomer and small oligomer self-assembly trends in the early stages of the nucleating process.

Mass Spectrometry as efficient tool for the characterization of amyloid peptide 25-35 self-assembly species in aggregation and inhibition studies

FIORI, JESSICA;NALDI, MARINA;ANDRISANO, VINCENZA
2013

Abstract

Amyloid beta 25-35 [A beta (25-35)], as a peptide model for full-Length A beta in structural and functional investigations, has been chosen for aggregation studies. The complexity of the A beta (25-35) aggregation process required a multi-methodological analytical approach to obtain reliable and reproducible results. Here, we describe the results obtained by the use of mass spectrometry (MS) for the structural characterization of the self-assembly species during the aggregation process and for the definition of the self-assembly kinetics and myricetin inhibition patterns, comparing the results with those obtained by using the well-established spectroscopic method based on thioflavin T fluorescence. Flow injection electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) was applied to monitor the disappearance of the monomer specie in the first steps, whereas matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-ToF-MS) was used to follow monomer and small oligomer self-assembly trends in the early stages of the nucleating process.
J. Fiori; M. Naldi; V. Andrisano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/256482
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