An increasing number of inter- and intramolecular interactions can nowadays be probed using single-molecule manipulation techniques. Protein unfolding and refolding is the most representative--though complex--of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single-molecule manipulation instrumentations: optical tweezers and atomic force microscopes.
Samorì B., Zuccheri G., Baschieri P. (2005). Protein unfolding and refolding under force: methodologies for nanomechanics. CHEMPHYSCHEM, 6 (1), 29-34 [10.1002/cphc.200400343].
Protein unfolding and refolding under force: methodologies for nanomechanics.
SAMORI', BRUNO;ZUCCHERI, GIAMPAOLO;
2005
Abstract
An increasing number of inter- and intramolecular interactions can nowadays be probed using single-molecule manipulation techniques. Protein unfolding and refolding is the most representative--though complex--of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single-molecule manipulation instrumentations: optical tweezers and atomic force microscopes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
