Aflatoxins are products of secondary metabolism of various species of Aspergillus, a fungus that grows in a wide range of food and animal feedstuffs. Aflatoxin B1 ingested by cows is epatically transformed in aflatoxin M1 (AfM1) and then secreted in milk. The European Union (EU) has established regulatory limits of the presence of AfM1 in milk and has suggested the methods of control: ELISA for screening and HPLC for confirmation. Hence the importance to constantly test the validity of analytical methods, not only on spiked samples but also on naturally contaminated matrices. In this work several trials have been conducted to evaluate the performances of two different commercial ELISA kits on naturally contaminated milk in the range from 10 ppt to 85 ppt; the samples were previously analysed by HPLC to determine the level of AfM1. Results showed good responses in both kits in terms of accuracy and precision.
Serraino A., Zironi E., Gazzotti T., Sticca P., B. Lugoboni, Pagliuca G. (2005). Comparison between two immunoenzimatic tests for determination of aflatoxin M1 concentration in naturally contaminated milk. CAGLIARI : Edizioni Kalb.
Comparison between two immunoenzimatic tests for determination of aflatoxin M1 concentration in naturally contaminated milk
SERRAINO, ANDREA;ZIRONI, ELISA;GAZZOTTI, TERESA;STICCA, PATRIZIA;LUGOBONI, BARBARA;PAGLIUCA, GIAMPIERO
2005
Abstract
Aflatoxins are products of secondary metabolism of various species of Aspergillus, a fungus that grows in a wide range of food and animal feedstuffs. Aflatoxin B1 ingested by cows is epatically transformed in aflatoxin M1 (AfM1) and then secreted in milk. The European Union (EU) has established regulatory limits of the presence of AfM1 in milk and has suggested the methods of control: ELISA for screening and HPLC for confirmation. Hence the importance to constantly test the validity of analytical methods, not only on spiked samples but also on naturally contaminated matrices. In this work several trials have been conducted to evaluate the performances of two different commercial ELISA kits on naturally contaminated milk in the range from 10 ppt to 85 ppt; the samples were previously analysed by HPLC to determine the level of AfM1. Results showed good responses in both kits in terms of accuracy and precision.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
