The TprI antigen of Treponema pallidum subsp. pallidum is a putative virulence factor predicted to be located in the outer membrane of the syphilis spirochete. In this study, we analyzed the immune response against TprI and its subunits in sera collected both from rabbits experimentally infected with the Nichols strain and from patients with syphilis, showing a different pattern of reactivity towards the antigen in these two groups of samples. Protective ability of recombinant TprI and its hypothetical outer membrane location were also investigated. Although no rabbit was protected after challenge, immunoelectronmicroscopy results, to be further investigated, were compatible with the outer membrane location of the antigen.
Giacani, L., Sambri, V., Marangoni, A., Cavrini, F., Storni, E., Donati, M., et al. (2005). Immunological evaluation and cellular location analysis of the TprI antigen of Treponema pallidum subspcies pallidum. INFECTION AND IMMUNITY, 73, 3817-3822 [10.1128/IAI.73.6.3817-3822.2005].
Immunological evaluation and cellular location analysis of the TprI antigen of Treponema pallidum subspcies pallidum
GIACANI, LORENZO;SAMBRI, VITTORIO;MARANGONI, ANTONELLA;CAVRINI, FRANCESCA;STORNI, ELISA;DONATI, MANUELA;CEVENINI, ROBERTO
2005
Abstract
The TprI antigen of Treponema pallidum subsp. pallidum is a putative virulence factor predicted to be located in the outer membrane of the syphilis spirochete. In this study, we analyzed the immune response against TprI and its subunits in sera collected both from rabbits experimentally infected with the Nichols strain and from patients with syphilis, showing a different pattern of reactivity towards the antigen in these two groups of samples. Protective ability of recombinant TprI and its hypothetical outer membrane location were also investigated. Although no rabbit was protected after challenge, immunoelectronmicroscopy results, to be further investigated, were compatible with the outer membrane location of the antigen.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
