Glycyrrhizin (1), the main active principle of Glycyrrhiza glabra (liquorice) roots, is extensively used in herbal medicines, in pharmaceutical preparations and confectionery products. A feasible and reliable method, which allows to simultaneously analyse 1 and its aglycone, glycyrrhetic acid (2), by means of an isocratic HPLC procedure is described. The system uses a C8 (150 x 4.6 mm) column as the stationary phase and a mixture of acetonitrile, methanol, water and glacial acetic acid as the mobile phase. Good linearity was found in the 1-50 µg/mL and in the 0.05 - 2.50 µg/mL concentration ranges for 1 and 2, respectively. A simple and rapid sample pretreatment, based on the extraction of the two analytes with a mixture of water and ethanol was implemented for the examined liquorice confectionery products and root samples. The HPLC method demonstrated to be suitable, in terms of precision and feasibility, for the quality control of the analytes in these matrices.
C. Sabbioni, A. Ferranti, F. Bugamelli, G. Cantelli Forti, M.A. Raggi (2006). Simultaneous HPLC analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and confectionery products. PHYTOCHEMICAL ANALYSIS, 17, 25-31 [10.1002/pca.877].
Simultaneous HPLC analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and confectionery products
SABBIONI, CESARE;FERRANTI, ANNA;BUGAMELLI, FRANCESCA;CANTELLI FORTI, GIORGIO;RAGGI, MARIA AUGUSTA
2006
Abstract
Glycyrrhizin (1), the main active principle of Glycyrrhiza glabra (liquorice) roots, is extensively used in herbal medicines, in pharmaceutical preparations and confectionery products. A feasible and reliable method, which allows to simultaneously analyse 1 and its aglycone, glycyrrhetic acid (2), by means of an isocratic HPLC procedure is described. The system uses a C8 (150 x 4.6 mm) column as the stationary phase and a mixture of acetonitrile, methanol, water and glacial acetic acid as the mobile phase. Good linearity was found in the 1-50 µg/mL and in the 0.05 - 2.50 µg/mL concentration ranges for 1 and 2, respectively. A simple and rapid sample pretreatment, based on the extraction of the two analytes with a mixture of water and ethanol was implemented for the examined liquorice confectionery products and root samples. The HPLC method demonstrated to be suitable, in terms of precision and feasibility, for the quality control of the analytes in these matrices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
