BACKGROUND: Histological and clinical criteria are generally used to differentiate second primary tumors (SPTs) from local recurrences. The purpose of the present study was to apply mitochondrial DNA (mtDNA) D-loop analysis to differentiate SPTs from local recurrences and to validate the clinical classification. METHODS: The study population consisted of 20 consecutive patients presenting multiple oral neoplastic lesions for a total of 25 paired lesions. The mtDNA D-loop analysis was performed by direct sequencing and phylogenetic clusterization. RESULTS: Agreement between mtDNA analysis and clinical classification was found in 19 cases. Discrepancies arose in 6 cases in which the clinical criteria based only on the spatial or temporal distance of the second lesion from the index tumor had led to a diagnosis of SPT (2 cases) or local recurrence (4 cases). CONCLUSION: The present data highlight the value of mtDNA analysis in establishing the clonal relationship between the index tumor and the second neoplastic lesion. © 2013 Wiley Periodicals, Inc. Head Neck 36: 94-100, 2014.
Tarsitano A, Leonardi E, Morandi L, Farnedi A, Montebugnoli L, Marchetti C (2014). LATE SKIP LYMPH NODE METASTASIS OF ORAL SQUAMOUS CELL CARCINOMA OR METASTASIS OF UNKNOWN SECOND PRIMARY TUMOR?ANSWER BY MITOCHONDRIAL DNA ANALYSIS. ORAL SURGERY, ORAL MEDICINE, ORAL PATHOLOGY AND ORAL RADIOLOGY, 117(1), 11-14 [10.1016/j.oooo.2012.02.038.].
LATE SKIP LYMPH NODE METASTASIS OF ORAL SQUAMOUS CELL CARCINOMA OR METASTASIS OF UNKNOWN SECOND PRIMARY TUMOR?ANSWER BY MITOCHONDRIAL DNA ANALYSIS
TARSITANO, ACHILLE;LEONARDI, ELISA;MORANDI, LUCA;FARNEDI, ANNA;MONTEBUGNOLI, LUCIO;MARCHETTI, CLAUDIO
2014
Abstract
BACKGROUND: Histological and clinical criteria are generally used to differentiate second primary tumors (SPTs) from local recurrences. The purpose of the present study was to apply mitochondrial DNA (mtDNA) D-loop analysis to differentiate SPTs from local recurrences and to validate the clinical classification. METHODS: The study population consisted of 20 consecutive patients presenting multiple oral neoplastic lesions for a total of 25 paired lesions. The mtDNA D-loop analysis was performed by direct sequencing and phylogenetic clusterization. RESULTS: Agreement between mtDNA analysis and clinical classification was found in 19 cases. Discrepancies arose in 6 cases in which the clinical criteria based only on the spatial or temporal distance of the second lesion from the index tumor had led to a diagnosis of SPT (2 cases) or local recurrence (4 cases). CONCLUSION: The present data highlight the value of mtDNA analysis in establishing the clonal relationship between the index tumor and the second neoplastic lesion. © 2013 Wiley Periodicals, Inc. Head Neck 36: 94-100, 2014.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
