The purposes of this study were to determine physiological changes occurring in hematological and biochemical parameters in mares between the last month of gestation and the first week after parturition. If a significant change was observed with respect to the reference interval of an adult horse, a further aim of the study was to establish different reference intervals. Blood samples were collected from n = 62 healthy pregnant Standardbred mares. Seventeen non-pregnant and non-lactating mares were used as control group. In pregnant mares, blood sampling was conducted every three days from one month before the expecting foaling date (335 days after the last mating), at parturition and seven days after foaling. The barren mares in the control group were collected once. Results from samples collected 20 days and 10 days before parturition, at parturition , and seven days after were considered for statistical analysis. A parametric method for all the parameters studied was used to establish reference intervals. Results were compared by repeated measures ANOVA. When significant differences were observed in relation to sampling time, a post hoc analysis was performed (Tukey test). The 1-way ANOVA test followed by Dunnett’s test was performed to evaluate the presence of a significant difference between each sampling time and the control group. Any significant difference in the blood count parameters at different sampling times were observed by repeated measure ANOVA. Hemoglobin (p <0.01) and hematocrit (p <0.01) seven days after parturition and white blood cell count (p <0.01) at parturition were significantly different from control group. Erythrocyte indices and platelet count were within the normal reference intervals as established in the control group. In the biochemical panel, gamma-glutamyltransferase, creatinine, glucose, biliar acids, total protein, albumin/globulin ratio, and calcium were significantly different at different sampling times. Moreover, serum concentration of creatine kinase, aspartate aminotransferase, creatinine, blood urea nitrogen, total, direct and indirect bilirubin, glucose, lactate, total protein, albumin, albumin/globulin ratio, calcium, magnesium, sodium, chloride, potassium, were different from control group. Remarkable changes were not observed in alkaline phosphatase, triglyceride and fibrinogen concentration. The temporal changes in the hematological and biochemical parameters observed in the present study in the peripartum and the differences with reference intervals made up for non-pregnant and non-lactating mares could be used to better evaluate the conditions of periparturient mares.
Mariella J., Pirrone A., Gentilini F., Castagnetti C. (2014). Hematologic and biochemical profiles in Standardbred mares during peripartum. THERIOGENOLOGY, 81(4), 526-534 [10.1016/j.theriogenology.2013.11.001].
Hematologic and biochemical profiles in Standardbred mares during peripartum
MARIELLA, JOLE;PIRRONE, ALESSANDRO;GENTILINI, FABIO;CASTAGNETTI, CAROLINA
2014
Abstract
The purposes of this study were to determine physiological changes occurring in hematological and biochemical parameters in mares between the last month of gestation and the first week after parturition. If a significant change was observed with respect to the reference interval of an adult horse, a further aim of the study was to establish different reference intervals. Blood samples were collected from n = 62 healthy pregnant Standardbred mares. Seventeen non-pregnant and non-lactating mares were used as control group. In pregnant mares, blood sampling was conducted every three days from one month before the expecting foaling date (335 days after the last mating), at parturition and seven days after foaling. The barren mares in the control group were collected once. Results from samples collected 20 days and 10 days before parturition, at parturition , and seven days after were considered for statistical analysis. A parametric method for all the parameters studied was used to establish reference intervals. Results were compared by repeated measures ANOVA. When significant differences were observed in relation to sampling time, a post hoc analysis was performed (Tukey test). The 1-way ANOVA test followed by Dunnett’s test was performed to evaluate the presence of a significant difference between each sampling time and the control group. Any significant difference in the blood count parameters at different sampling times were observed by repeated measure ANOVA. Hemoglobin (p <0.01) and hematocrit (p <0.01) seven days after parturition and white blood cell count (p <0.01) at parturition were significantly different from control group. Erythrocyte indices and platelet count were within the normal reference intervals as established in the control group. In the biochemical panel, gamma-glutamyltransferase, creatinine, glucose, biliar acids, total protein, albumin/globulin ratio, and calcium were significantly different at different sampling times. Moreover, serum concentration of creatine kinase, aspartate aminotransferase, creatinine, blood urea nitrogen, total, direct and indirect bilirubin, glucose, lactate, total protein, albumin, albumin/globulin ratio, calcium, magnesium, sodium, chloride, potassium, were different from control group. Remarkable changes were not observed in alkaline phosphatase, triglyceride and fibrinogen concentration. The temporal changes in the hematological and biochemical parameters observed in the present study in the peripartum and the differences with reference intervals made up for non-pregnant and non-lactating mares could be used to better evaluate the conditions of periparturient mares.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
