A procedure for the determination of Imidacloprid and its main metabolites was set up by means of liquid chromatography with an electrochemical detector and post-column photochemical reactor (LC-h-ED). Sample clean-up was developed for bees, filter paper and maize leaves. Chromatographic conditions were based on a reversed-phase C-18 column operated by phosphate buffer 50 mM/CH3CN (80/20, v/v) at pH 2.9. Determination of Imidacloprid and its metabolites was carried out at a potential of 800 mV after photoactivation at 254 nm. Compared to conventional techniques such as gas-chromatography/mass-spectrometry (GC/MS) or LC coupled to other detectors, the present method allows simultaneous trace-level determination of both Imidacloprid (0.6 ng ml-1) and its main metabolites (2.4 ng ml-1).

Determination of Imidacloprid and metabolites by liquid chromatography with an electrochemical detector and post column photochemical reactor

GALLETTI, GUIDO
2006

Abstract

A procedure for the determination of Imidacloprid and its main metabolites was set up by means of liquid chromatography with an electrochemical detector and post-column photochemical reactor (LC-h-ED). Sample clean-up was developed for bees, filter paper and maize leaves. Chromatographic conditions were based on a reversed-phase C-18 column operated by phosphate buffer 50 mM/CH3CN (80/20, v/v) at pH 2.9. Determination of Imidacloprid and its metabolites was carried out at a potential of 800 mV after photoactivation at 254 nm. Compared to conventional techniques such as gas-chromatography/mass-spectrometry (GC/MS) or LC coupled to other detectors, the present method allows simultaneous trace-level determination of both Imidacloprid (0.6 ng ml-1) and its main metabolites (2.4 ng ml-1).
ANALYTICA CHIMICA ACTA
M. Rancan; A.G. Sabatini; G. Achilli; G.C. Galletti
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/20617
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