A procedure for the determination of Imidacloprid and its main metabolites was set up by means of liquid chromatography with an electrochemical detector and post-column photochemical reactor (LC-h-ED). Sample clean-up was developed for bees, filter paper and maize leaves. Chromatographic conditions were based on a reversed-phase C-18 column operated by phosphate buffer 50 mM/CH3CN (80/20, v/v) at pH 2.9. Determination of Imidacloprid and its metabolites was carried out at a potential of 800 mV after photoactivation at 254 nm. Compared to conventional techniques such as gas-chromatography/mass-spectrometry (GC/MS) or LC coupled to other detectors, the present method allows simultaneous trace-level determination of both Imidacloprid (0.6 ng ml-1) and its main metabolites (2.4 ng ml-1).
M. Rancan, A.G. Sabatini, G. Achilli, G.C. Galletti (2006). Determination of Imidacloprid and metabolites by liquid chromatography with an electrochemical detector and post column photochemical reactor. ANALYTICA CHIMICA ACTA, 555, 20-24 [10.1016/j.aca.2005.08.058].
Determination of Imidacloprid and metabolites by liquid chromatography with an electrochemical detector and post column photochemical reactor
GALLETTI, GUIDO
2006
Abstract
A procedure for the determination of Imidacloprid and its main metabolites was set up by means of liquid chromatography with an electrochemical detector and post-column photochemical reactor (LC-h-ED). Sample clean-up was developed for bees, filter paper and maize leaves. Chromatographic conditions were based on a reversed-phase C-18 column operated by phosphate buffer 50 mM/CH3CN (80/20, v/v) at pH 2.9. Determination of Imidacloprid and its metabolites was carried out at a potential of 800 mV after photoactivation at 254 nm. Compared to conventional techniques such as gas-chromatography/mass-spectrometry (GC/MS) or LC coupled to other detectors, the present method allows simultaneous trace-level determination of both Imidacloprid (0.6 ng ml-1) and its main metabolites (2.4 ng ml-1).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
