On-chip optical detection of chemiluminescent reactions is presented. The device is based on the integration of thin film hydrogenated amorphous silicon photosensors on a functionalized glass substrate ensuring both a good optical coupling and an optimal separation between biological or chemical reagents and the sensing elements. The sensor has been characterized and optimized using the chemiluminescent system composed by the enzyme horseradish peroxidase (HRP) and luminol/peroxide/enhancer cocktail. The detectability of HRP is at the attomole level with a sensitivity of 1.46 fA/fg. Experiments, involving the detection of immobilized bio-specific probes on the functionalized surface have been performed both in bulk and microfluidics regime, proving the ability of the system to effectively detect chemiluminescent reactions and their kinetics. In particular, results achieved using conventional polydimethylsiloxane microfluidics for samples and reagents handling confirmed the good detection capabilities of the proposed system.

Domenico Caputo, Giampiero de Cesare, Luisa Stella Dolci, Mara Mirasoli, Augusto Nascetti, Aldo Roda, et al. (2013). Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays. IEEE SENSORS JOURNAL, 13, 2595-2602 [10.1109/JSEN.2013.2256889].

Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays

DOLCI, LUISA STELLA;MIRASOLI, MARA;RODA, ALDO;
2013

Abstract

On-chip optical detection of chemiluminescent reactions is presented. The device is based on the integration of thin film hydrogenated amorphous silicon photosensors on a functionalized glass substrate ensuring both a good optical coupling and an optimal separation between biological or chemical reagents and the sensing elements. The sensor has been characterized and optimized using the chemiluminescent system composed by the enzyme horseradish peroxidase (HRP) and luminol/peroxide/enhancer cocktail. The detectability of HRP is at the attomole level with a sensitivity of 1.46 fA/fg. Experiments, involving the detection of immobilized bio-specific probes on the functionalized surface have been performed both in bulk and microfluidics regime, proving the ability of the system to effectively detect chemiluminescent reactions and their kinetics. In particular, results achieved using conventional polydimethylsiloxane microfluidics for samples and reagents handling confirmed the good detection capabilities of the proposed system.
2013
Domenico Caputo, Giampiero de Cesare, Luisa Stella Dolci, Mara Mirasoli, Augusto Nascetti, Aldo Roda, et al. (2013). Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays. IEEE SENSORS JOURNAL, 13, 2595-2602 [10.1109/JSEN.2013.2256889].
Domenico Caputo;Giampiero de Cesare;Luisa Stella Dolci;Mara Mirasoli;Augusto Nascetti;Aldo Roda;Riccardo Scipinotti
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/191372
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 40
  • ???jsp.display-item.citation.isi??? 34
social impact