Single layer centrifugation (SLC) is a useful technique to select porcine spermatozoa for further artificial insemination practices. The aim of this study was to determine possible side-effects related to capacitation due to the process. Semen viability, acrosome integrity and capacitation status were determined through fluorescent probes (SYBR14-PI, FITCPSA, CTC staining) and Hsp70 immunolocalization and protein tyrosine phosphorylation (by western blotting and immunolocalization) in different groups: control, after SLC with Androcoll (AND), after SLC and washing (AND-Wash) and after SLC, washing and storage for 2 h at 17 ◦C with 2.5% of seminal plasma (AND-Wash-SP). Neither viability nor acrosome integrity were impaired by the different treatments; as far as CTC staining, we observed a significant increase (p < 0.05) in the capacitation related pattern in AND and AND-Wash, while after exposure for 2 h to seminal plasma (AND-Wash-SP group), the increase became less evident; the same trend was observed in Hsp70 immunolocalization for the EL pattern. Neither immunolocalization nor western blotting for tyrosine phosphorylated proteins had an increase in capacitated pattern or in phosphorylation status, except for a 25 kDa band that increased in AND and AND-Wash groups and decreased in AND-Wash-SP group. SLC using Androcoll-P induces some capacitation-related changes in boar sperm membrane, as demonstrated by CTC staining and Hsp70 immunolocalization. For protein tyrosine phosphorylation, only a 25 kDa protein showed some changes that should be investigated further.

Diego Bucci, Marcella Spinaci, Jane Morrell, Claudia Vallorani, Carlo Tamanini, Rita Guidetti, et al. (2013). Effects of single layer centrifugation with Androcoll-P on boar sperm. ANIMAL REPRODUCTION SCIENCE, 138, 276-281 [10.1016/j.anireprosci.2013.02.020].

Effects of single layer centrifugation with Androcoll-P on boar sperm

BUCCI, DIEGO;SPINACI, MARCELLA;VALLORANI, CLAUDIA;TAMANINI, CARLO;GALEATI, GIOVANNA
2013

Abstract

Single layer centrifugation (SLC) is a useful technique to select porcine spermatozoa for further artificial insemination practices. The aim of this study was to determine possible side-effects related to capacitation due to the process. Semen viability, acrosome integrity and capacitation status were determined through fluorescent probes (SYBR14-PI, FITCPSA, CTC staining) and Hsp70 immunolocalization and protein tyrosine phosphorylation (by western blotting and immunolocalization) in different groups: control, after SLC with Androcoll (AND), after SLC and washing (AND-Wash) and after SLC, washing and storage for 2 h at 17 ◦C with 2.5% of seminal plasma (AND-Wash-SP). Neither viability nor acrosome integrity were impaired by the different treatments; as far as CTC staining, we observed a significant increase (p < 0.05) in the capacitation related pattern in AND and AND-Wash, while after exposure for 2 h to seminal plasma (AND-Wash-SP group), the increase became less evident; the same trend was observed in Hsp70 immunolocalization for the EL pattern. Neither immunolocalization nor western blotting for tyrosine phosphorylated proteins had an increase in capacitated pattern or in phosphorylation status, except for a 25 kDa band that increased in AND and AND-Wash groups and decreased in AND-Wash-SP group. SLC using Androcoll-P induces some capacitation-related changes in boar sperm membrane, as demonstrated by CTC staining and Hsp70 immunolocalization. For protein tyrosine phosphorylation, only a 25 kDa protein showed some changes that should be investigated further.
2013
Diego Bucci, Marcella Spinaci, Jane Morrell, Claudia Vallorani, Carlo Tamanini, Rita Guidetti, et al. (2013). Effects of single layer centrifugation with Androcoll-P on boar sperm. ANIMAL REPRODUCTION SCIENCE, 138, 276-281 [10.1016/j.anireprosci.2013.02.020].
Diego Bucci; Marcella Spinaci; Jane Morrell; Claudia Vallorani; Carlo Tamanini; Rita Guidetti; Giovanna Galeati
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/187737
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact