A kinetic study of butane uptake and trichloroethylene (TCE) aerobic cometabolism was conducted by two suspended-cell (15 and 30°C) and two attached-cell (15 and 30°C) consortia obtained from the indigenous biomass of a TCE-contaminated aquifer. The shift from suspended to attached cells resulted in an increase of butane (15 and 30°C) and TCE (15°C) biodegradation rates, and a significant decrease of butane inhibition on TCE biodegradation. The TCE 15°C maximum specific biodegradation rate was equal to 0.011 mg TCE/mg protein/d with suspended cells and 0.021 mg TCE/mg protein/d with attached cells. The type of mutual butane/TCE inhibition depended on temperature and biomass conditions. On the basis of a continuous-flow simulation, a packed-bed PFR inoculated with the 15°C or 30°C attached-cell consortium could attain a 99.96% conversion of the studied site’s average TCE concentration with a 0.4-0.5-day hydraulic residence time, with a low effect of temperature on the TCE degradation performances.

Trichloroethylene aerobic cometabolism by suspended and immobilized butane-growing microbial consortia: A kinetic study

FRASCARI, DARIO;ZANAROLI, GIULIO;BUCCHI, GIACOMO;ROSATO, ANTONELLA;TAVANAIE, NASRIN;FRARACCIO, SERENA;PINELLI, DAVIDE;FAVA, FABIO
2013

Abstract

A kinetic study of butane uptake and trichloroethylene (TCE) aerobic cometabolism was conducted by two suspended-cell (15 and 30°C) and two attached-cell (15 and 30°C) consortia obtained from the indigenous biomass of a TCE-contaminated aquifer. The shift from suspended to attached cells resulted in an increase of butane (15 and 30°C) and TCE (15°C) biodegradation rates, and a significant decrease of butane inhibition on TCE biodegradation. The TCE 15°C maximum specific biodegradation rate was equal to 0.011 mg TCE/mg protein/d with suspended cells and 0.021 mg TCE/mg protein/d with attached cells. The type of mutual butane/TCE inhibition depended on temperature and biomass conditions. On the basis of a continuous-flow simulation, a packed-bed PFR inoculated with the 15°C or 30°C attached-cell consortium could attain a 99.96% conversion of the studied site’s average TCE concentration with a 0.4-0.5-day hydraulic residence time, with a low effect of temperature on the TCE degradation performances.
BIORESOURCE TECHNOLOGY
Dario Frascari;Giulio Zanaroli;Giacomo Bucchi;Antonella Rosato;Nasrin Tavanaie;Serena Fraraccio;Davide Pinelli;Fabio Fava
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/185707
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 24
social impact