The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker. Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR. These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.
Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum / Sadeghinezhad J; Sorteni C; Di Guardo G; D’Agostino C; Agrimi U; Nonno R; Chiocchetti R. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - ELETTRONICO. - 95:3(2013), pp. 846-853. [10.1016/j.rvsc.2013.07.028]
Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum
SADEGHINEZHAD, JAVAD;SORTENI, CATERINA;CHIOCCHETTI, ROBERTO
2013
Abstract
The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker. Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR. These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
