Hydrangea species are infected by phytoplasmas belonging to subgroups 16SrI-A and 16SrI-B (aster yellows) inducing phyllody and virescence worldwide. In Japan some of the phytoplasma associated such disease were classified as ‘Candidatus Phytoplasma japonicum’, moreover 16SrXII-A phytoplasmas were identified in Bulgaria and more recently in Italy. The hydrangea phyllody disease was associated with 16SrI-B phytoplasmas in Canada, however no fine molecular characterization was carried out on the phytoplasmas in that country. Hydrangeas showing symptoms similar to those associated with phytoplasma diseases were found and collected from landscape areas in South-western Ontario, Canada. Phytoplasmas belonging aster yellows group were detected in all symptomatic samples tested by two different methodologies. Direct sequencing and sequence obtained after cloning of the 16Sr DNA gene show confirmation of reported classification and the amplification and sequencing of groEL gene confirm previous classification in subgroup 16SrI-B and allow to enclose aster yellows phytoplasmas in hydrangeas from Canada in the groELI-III group that up to now was only detected in Europe.
Duduk B., Y. Arocha, J. Mitrović, R. Michelutti, R. Benabid, J. Scott, et al. (2013). Caratterizzazione molecolare di ‘Candidatus Phytoplasma asteris’ in ortensia in Canada. PETRIA, 23(1), 139-142.
Caratterizzazione molecolare di ‘Candidatus Phytoplasma asteris’ in ortensia in Canada
BERTACCINI, ASSUNTA
2013
Abstract
Hydrangea species are infected by phytoplasmas belonging to subgroups 16SrI-A and 16SrI-B (aster yellows) inducing phyllody and virescence worldwide. In Japan some of the phytoplasma associated such disease were classified as ‘Candidatus Phytoplasma japonicum’, moreover 16SrXII-A phytoplasmas were identified in Bulgaria and more recently in Italy. The hydrangea phyllody disease was associated with 16SrI-B phytoplasmas in Canada, however no fine molecular characterization was carried out on the phytoplasmas in that country. Hydrangeas showing symptoms similar to those associated with phytoplasma diseases were found and collected from landscape areas in South-western Ontario, Canada. Phytoplasmas belonging aster yellows group were detected in all symptomatic samples tested by two different methodologies. Direct sequencing and sequence obtained after cloning of the 16Sr DNA gene show confirmation of reported classification and the amplification and sequencing of groEL gene confirm previous classification in subgroup 16SrI-B and allow to enclose aster yellows phytoplasmas in hydrangeas from Canada in the groELI-III group that up to now was only detected in Europe.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
