“Flavescence dorée” (FD) is a disease still of relevant importance considering the ability of phytoplasmas associated with it to differentiate new strains in short periods of time. The first finding of FD in Tuscany dates back to 2003 and concerns a vineyard sited at Montignoso, in Massa Carrara province. In the last decade the monitoring of phytoplasma has highlighted a fairly widespread presence of the disease, only in the wine-growing areas of the provinces of Massa Carrara and Lucca. In the context of the program EUPHRESCO GRAFDEPI, in 2012 surveys were carried out mainly in vineyards in the province of Massa Carrara. The vineyard reported was monitored in 2009 as well, it is made of six varieties, four native (‘Albarola’, ‘Ciliegiolo’, ‘Pollera Pontremolese’ and ‘Vermentino’) plus ‘Chardonnay’ and ‘Merlot’, all grafted on SO4 rootstock. The flora of the border is made up by chestnut plants and also several shrubs including, blackberries, hawthorn, plum, elderberry and especially greybeard. A total of 30 samples of leaves and branches were collected from symptomatic vines (nine in 2009 and 21 in 2012) and a total of six samples of Clematis vitalba were collected from border flora in 2012. All grapevine samples positive to phytoplasmas (7/9 in 2009 and 14/21 in 2012) resulted infected by FD-C. Regarding to the greybeard a sample resulted positive and infected by 16SrXII-A (“stolbur”) phytoplasmas. Molecular characterization was carried out on FD strains identify that all the samples were infected by FD-D phytoplasmas, however the fine characterization on the SecY gene by RFLP analyses showed that all strains but one have a profile indistinguishable from the one typically described in FD-C strains. One sample showed profile mixed between typical FD-C and FD-D strains. This result indicates that also in Tuscany FD-D phytoplasma is producing strains as already reported in other Italian FD-D epidemic as well as in one strain from France. This finding is quite important since the possibility to early identify new FD strains allows to better manage the disease before these strains become epidemically relevant.
Variabilità genetica di fitoplasmi associati a flavescenza dorata in un vigneto dellaprovincia di Massa Carrara
BERTACCINI, ASSUNTA;PALTRINIERI, SAMANTA;CONTALDO, NICOLETTA;
2013
Abstract
“Flavescence dorée” (FD) is a disease still of relevant importance considering the ability of phytoplasmas associated with it to differentiate new strains in short periods of time. The first finding of FD in Tuscany dates back to 2003 and concerns a vineyard sited at Montignoso, in Massa Carrara province. In the last decade the monitoring of phytoplasma has highlighted a fairly widespread presence of the disease, only in the wine-growing areas of the provinces of Massa Carrara and Lucca. In the context of the program EUPHRESCO GRAFDEPI, in 2012 surveys were carried out mainly in vineyards in the province of Massa Carrara. The vineyard reported was monitored in 2009 as well, it is made of six varieties, four native (‘Albarola’, ‘Ciliegiolo’, ‘Pollera Pontremolese’ and ‘Vermentino’) plus ‘Chardonnay’ and ‘Merlot’, all grafted on SO4 rootstock. The flora of the border is made up by chestnut plants and also several shrubs including, blackberries, hawthorn, plum, elderberry and especially greybeard. A total of 30 samples of leaves and branches were collected from symptomatic vines (nine in 2009 and 21 in 2012) and a total of six samples of Clematis vitalba were collected from border flora in 2012. All grapevine samples positive to phytoplasmas (7/9 in 2009 and 14/21 in 2012) resulted infected by FD-C. Regarding to the greybeard a sample resulted positive and infected by 16SrXII-A (“stolbur”) phytoplasmas. Molecular characterization was carried out on FD strains identify that all the samples were infected by FD-D phytoplasmas, however the fine characterization on the SecY gene by RFLP analyses showed that all strains but one have a profile indistinguishable from the one typically described in FD-C strains. One sample showed profile mixed between typical FD-C and FD-D strains. This result indicates that also in Tuscany FD-D phytoplasma is producing strains as already reported in other Italian FD-D epidemic as well as in one strain from France. This finding is quite important since the possibility to early identify new FD strains allows to better manage the disease before these strains become epidemically relevant.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
