Bermuda grass white leaf (BGWL) is a destructive disease of bermuda grass (Cynodon dactylon L.) associated with the presence of ‘Candidatus Phytoplasma cynodontis’. Strains belonging to 16SrXIV bermuda grass white leaf group were detected in several tropical and subtropical areas worldwide and regions bordering to these climates. BGWL strains can be separated by genetic markers from economical important phylogenetic-related strains such as sugarcane white leaf (SCWL), sugarcane grassy shoot (SCGS), rice yellow dwarf (RYD) and sorghum grassy shoot (SGS) phytoplasmas. Beside this interesting relationship, BGWL was described carry the smallest chromosome in phytoplasmas. Little is known about the genomes of BGWL strains and the coding of effectors or membrane proteins probable interacting with host and/or vector. In a genomic survey, a first draft sequence of a BGWL from Italy was determined. Preliminary analysis of the gene content highlighted the coding of an imp-gene fragment. The imp-region of three samples from Italy was reconstructed by a PCR-approach using four primers derived from the genomic draft. The impregion of these samples, above 5 kb in size, encodes a dsRNA-specific ribonuclease RNaseIII (Rnc), a chromosome replication initiation protein (DnaD), imp-like protein (Imp), a CTP synthase (PyrG), probable a phosphatidylserine decarboxylase (psd) and a phosphatidylserine synthase (PssA). Gene order of this region is identical to ‘Candidatus Phytoplasma oryzae’ (acc. no. AB469012) derived from a Japanese strain detected in Farfugium japonicum var. Kitamura. Partial sequences of the 5’- end of imp were determined for six samples showing no variation in their nucleotide sequence to each other indicating an unexpected conservation of this gene encoding a putative membrane protein in BGWL strains.
Organizzazione del gene imp in ‘Candidatus Phytoplasma cynodontis’
PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2013
Abstract
Bermuda grass white leaf (BGWL) is a destructive disease of bermuda grass (Cynodon dactylon L.) associated with the presence of ‘Candidatus Phytoplasma cynodontis’. Strains belonging to 16SrXIV bermuda grass white leaf group were detected in several tropical and subtropical areas worldwide and regions bordering to these climates. BGWL strains can be separated by genetic markers from economical important phylogenetic-related strains such as sugarcane white leaf (SCWL), sugarcane grassy shoot (SCGS), rice yellow dwarf (RYD) and sorghum grassy shoot (SGS) phytoplasmas. Beside this interesting relationship, BGWL was described carry the smallest chromosome in phytoplasmas. Little is known about the genomes of BGWL strains and the coding of effectors or membrane proteins probable interacting with host and/or vector. In a genomic survey, a first draft sequence of a BGWL from Italy was determined. Preliminary analysis of the gene content highlighted the coding of an imp-gene fragment. The imp-region of three samples from Italy was reconstructed by a PCR-approach using four primers derived from the genomic draft. The impregion of these samples, above 5 kb in size, encodes a dsRNA-specific ribonuclease RNaseIII (Rnc), a chromosome replication initiation protein (DnaD), imp-like protein (Imp), a CTP synthase (PyrG), probable a phosphatidylserine decarboxylase (psd) and a phosphatidylserine synthase (PssA). Gene order of this region is identical to ‘Candidatus Phytoplasma oryzae’ (acc. no. AB469012) derived from a Japanese strain detected in Farfugium japonicum var. Kitamura. Partial sequences of the 5’- end of imp were determined for six samples showing no variation in their nucleotide sequence to each other indicating an unexpected conservation of this gene encoding a putative membrane protein in BGWL strains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
