Mesotheliomas are rare in the cat with only ten previously reported cases in the veterinary literature, affecting mostly the peritoneum. This paper describes another ten cases (5 females and 5 males; age range 1-17 years; 8 pleural and 2 peritoneal). Histologically, 5 tumours were epithelial, 3 fibrosarcomatous and 2 biphasic. Several immunohistochemical markers have been suggested in human pathology as useful markers for the identification of mesothelioma: vimentin, cytokeratin (CK) AE1/AE3, HBME-1, CK 5/6, calretinin, thrombomodulin, carcinoembryonic antigen (CEA), CD15, E-cadherin and desmin. On the basis of this, antibodies CK 5/6, HBME-1, calretinin, thrombomodulin and CD15 have been tested in the cat for the first time. All cases were positive for both vimentin and CK AE1/AE3, 6 cases for HBME-1, 2 cases for CK 5/6, 3 cases for CEA and 4 cases for E-Cadherin. All cases were negative for desmin, calretinin. Thrombomodulin and CD15 did not cross-react with feline tissues. Electron microscopy was performed in 4 cases, revealing the presence of microvillar structures, desmosomes and intracytoplasmic lumina, confirming its utility as diagnostic tool. The results presented show that mesothelial markers commonly used in humans can be used for the diagnosis of feline mesothelioma, preferably a panel of antibodies rather than a single one.
Ten Cases of Feline Mesothelioma: an Immunohistochemical and Ultrastructural Study
BACCI, BARBARA;MORANDI, FEDERICO;MARCATO, PAOLO STEFANO
2006
Abstract
Mesotheliomas are rare in the cat with only ten previously reported cases in the veterinary literature, affecting mostly the peritoneum. This paper describes another ten cases (5 females and 5 males; age range 1-17 years; 8 pleural and 2 peritoneal). Histologically, 5 tumours were epithelial, 3 fibrosarcomatous and 2 biphasic. Several immunohistochemical markers have been suggested in human pathology as useful markers for the identification of mesothelioma: vimentin, cytokeratin (CK) AE1/AE3, HBME-1, CK 5/6, calretinin, thrombomodulin, carcinoembryonic antigen (CEA), CD15, E-cadherin and desmin. On the basis of this, antibodies CK 5/6, HBME-1, calretinin, thrombomodulin and CD15 have been tested in the cat for the first time. All cases were positive for both vimentin and CK AE1/AE3, 6 cases for HBME-1, 2 cases for CK 5/6, 3 cases for CEA and 4 cases for E-Cadherin. All cases were negative for desmin, calretinin. Thrombomodulin and CD15 did not cross-react with feline tissues. Electron microscopy was performed in 4 cases, revealing the presence of microvillar structures, desmosomes and intracytoplasmic lumina, confirming its utility as diagnostic tool. The results presented show that mesothelial markers commonly used in humans can be used for the diagnosis of feline mesothelioma, preferably a panel of antibodies rather than a single one.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
