Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of sphericalpolyhedral shape cells. The luteinization index (progesterone:17-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

FOLLICLE-LIKE MODEL BY GRANULOSA CELL ENCAPSULATION IN A BARIUM ALGINATE/PROTAMINE MEMBRANE.

ACCORSI, PIER ATTILIO;GALEATI, GIOVANNA;SPINACI, MARCELLA;
2005

Abstract

Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of sphericalpolyhedral shape cells. The luteinization index (progesterone:17-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
VIGO D.; VILLANI S.; FAUSTINI M.; ACCORSI P.A.; GALEATI G.; SPINACI M.; MUNARI E.; RUSSO V.; ASTI A.; CONTE U.; TORRE ML.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/1563
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