Background: histological and clinical criteria are generally utilized to differentiate second primary tumors (SPT) from local recurrences (LR).. The purpose of the present study was to apply mtDNA D-loop analysis to differentiate SPT from LR and to validate the clinical classification. Methods: The study population consisted of 20 consecutive patients presenting multiple oral neoplastic lesions for a total of 25 paired lesions. mtDNA D-loop analysis was performed by direct sequencing and phylogenetic clusterization. Results: Agreement between mtDNA analysis and clinical classification was found in 19 cases. Discrepancies arose in 6 cases in which the clinical criteria based only on the spatial or temporal distance of the second lesion from the index tumor had led to a diagnosis of SPT (2 cases) or LR (4 cases). Conclusions: The present data highlight the value of mtDNA analysis in establishing the clonal relationship between the index tumor and the second neoplastic lesion.
Montebugnoli L, Leonardi E, Morandi L, Farnedi A, Gissi DB, Marchetti C, et al. (2014). Genetic relationship between multiple squamous cell carcinomas arising in the oral cavity. HEAD & NECK, 36(1), 94-100 [10.1002/hed.23259].
Genetic relationship between multiple squamous cell carcinomas arising in the oral cavity
MONTEBUGNOLI, LUCIO;MORANDI, LUCA;GISSI, DAVIDE BARTOLOMEO;MARCHETTI, CLAUDIO;TARSITANO, ACHILLE;FOSCHINI, MARIA PIA
2014
Abstract
Background: histological and clinical criteria are generally utilized to differentiate second primary tumors (SPT) from local recurrences (LR).. The purpose of the present study was to apply mtDNA D-loop analysis to differentiate SPT from LR and to validate the clinical classification. Methods: The study population consisted of 20 consecutive patients presenting multiple oral neoplastic lesions for a total of 25 paired lesions. mtDNA D-loop analysis was performed by direct sequencing and phylogenetic clusterization. Results: Agreement between mtDNA analysis and clinical classification was found in 19 cases. Discrepancies arose in 6 cases in which the clinical criteria based only on the spatial or temporal distance of the second lesion from the index tumor had led to a diagnosis of SPT (2 cases) or LR (4 cases). Conclusions: The present data highlight the value of mtDNA analysis in establishing the clonal relationship between the index tumor and the second neoplastic lesion.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
