Effects of Naturai Extract of Chestnut Wood in guinea pig ileum and proximal colon smooth muscle M. Micacei1. R.Budriesi1, P.loan1, A. Chiarini1, S. Giorgini2 1Dip. Scienze Farmaceutiche, Università di Bologna, via Bel meloro 6, 40126 Bologna, Italia; 2 Orogel S.p.A., Via Dismano, 2600, 47522 Cesena (FC), Italia matteo.micucci2@unibo.it Naturai Extract of Chestnut Wood (ENC) is widely used, in Folk Medicine, in thè treatment of gastroenteritis, diarrhoea and dysentery.1 In order to explain thè pharmacological role of tannins in thè modulation of intestina! motìlity, we studied the effects of purified ENC, which is rich in hydrolysable tannins, using biological in vitro assays. Isolated guinea pig ileum and proximal colon segments were used to evaluate the ability of the extract to inhibit contractions evoked by several agonists such as carbachol (CCh), serotonin (5-hydroxytryptamine [5-H7]), BaCI2, KCI, and histamìne. In guinea pig ileum, ENC has been shown to reduce thè maximum contraction induced by carbachol in a concentration-dependent manner, showing a non competitive antagonism towards thè muscarinic receptors. In addition, this activity has been demonstrated to be reversible, as thè response to CCh was completely recovered after 60 minutes of tissue washout. The antispasmodic activity of ENC was further investigated against a variety of different spasmogenic agents in the guinea pig ileum. ENC reduced the histamine-induced spasms through a non competitive mechanism, and the inhibition was completely reversed after 60 minutes of tissue wash out. In addition, pretreatment with ENC decreased thè contraction evoked by KCI and spasmodic contractions elicited by BaCI2 were reduced by ENC in a concentration-dependent manner. Also in guinea pig proximal colon, ENC showed a non competitive antagonism towards thè muscarinic receptors which has been observed to be reversible and to occur in a concentration-dependent manner. ENC is able to reduce gut peristalsis through several mechanisms of action.1 ENC antispasmodic effects, together with its antiviral and antibacterial activity against many food-borne pathogen bacteria, may be useful in the prevention and treatment of several gastro-intestinal disorders. References: Budriesi R. loan P. Micucci M. Micucci E. Lìmonoelli V. Chiarini A.. 2010. Stop Fitan: antispasmodic effect of naturai extract of chestnut wood in guinea pig ileum and proximal colon smooth muscle. J Med Food., 13 (5), 1104-10.
Micucci M, Budriesi R, Ioan P, Chiarini A, Giorgini S (2012). Effects of natural extract of chestnut wood in guinea pig ileum and proximal colon.. s.l. : s.n..
Effects of natural extract of chestnut wood in guinea pig ileum and proximal colon.
MICUCCI, MATTEO;BUDRIESI, ROBERTA;IOAN, PIERFRANCO;CHIARINI, ALBERTO;
2012
Abstract
Effects of Naturai Extract of Chestnut Wood in guinea pig ileum and proximal colon smooth muscle M. Micacei1. R.Budriesi1, P.loan1, A. Chiarini1, S. Giorgini2 1Dip. Scienze Farmaceutiche, Università di Bologna, via Bel meloro 6, 40126 Bologna, Italia; 2 Orogel S.p.A., Via Dismano, 2600, 47522 Cesena (FC), Italia matteo.micucci2@unibo.it Naturai Extract of Chestnut Wood (ENC) is widely used, in Folk Medicine, in thè treatment of gastroenteritis, diarrhoea and dysentery.1 In order to explain thè pharmacological role of tannins in thè modulation of intestina! motìlity, we studied the effects of purified ENC, which is rich in hydrolysable tannins, using biological in vitro assays. Isolated guinea pig ileum and proximal colon segments were used to evaluate the ability of the extract to inhibit contractions evoked by several agonists such as carbachol (CCh), serotonin (5-hydroxytryptamine [5-H7]), BaCI2, KCI, and histamìne. In guinea pig ileum, ENC has been shown to reduce thè maximum contraction induced by carbachol in a concentration-dependent manner, showing a non competitive antagonism towards thè muscarinic receptors. In addition, this activity has been demonstrated to be reversible, as thè response to CCh was completely recovered after 60 minutes of tissue washout. The antispasmodic activity of ENC was further investigated against a variety of different spasmogenic agents in the guinea pig ileum. ENC reduced the histamine-induced spasms through a non competitive mechanism, and the inhibition was completely reversed after 60 minutes of tissue wash out. In addition, pretreatment with ENC decreased thè contraction evoked by KCI and spasmodic contractions elicited by BaCI2 were reduced by ENC in a concentration-dependent manner. Also in guinea pig proximal colon, ENC showed a non competitive antagonism towards thè muscarinic receptors which has been observed to be reversible and to occur in a concentration-dependent manner. ENC is able to reduce gut peristalsis through several mechanisms of action.1 ENC antispasmodic effects, together with its antiviral and antibacterial activity against many food-borne pathogen bacteria, may be useful in the prevention and treatment of several gastro-intestinal disorders. References: Budriesi R. loan P. Micucci M. Micucci E. Lìmonoelli V. Chiarini A.. 2010. Stop Fitan: antispasmodic effect of naturai extract of chestnut wood in guinea pig ileum and proximal colon smooth muscle. J Med Food., 13 (5), 1104-10.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
