The presence of phytoplasmas in strawberry showing malformation of the fruits together with the typical green petals symptoms was detected in some North Western Italy cultivations. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rDNA and GroEL sequences specific for phytoplasmas. Bands of 1.2 kb were obtained in both cases after nested-PCR assays and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows subgroup 16SrI-C, the GroELI grouping confirm all the strains from strawberry to be identical to each other and to GroELI-VI group. This is the first multigene molecular identification of strawberry green petals phytoplasmas in Italy.

Identification and GroEL gene characterization of green petal phytoplasma infecting strawberry in Italy / Contaldo N.; J.F. Mejia; S. Paltrinieri; A. Calari; A. Bertaccini. - In: PHYTOPATHOGENIC MOLLICUTES. - ISSN 2249-4669. - STAMPA. - 2:2(2012), pp. 59-62. [10.5958/j.2249-4669.2.2.009]

Identification and GroEL gene characterization of green petal phytoplasma infecting strawberry in Italy

CONTALDO, NICOLETTA;MEJIA DE LOS RIOS, JUAN FERNANDO;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2012

Abstract

The presence of phytoplasmas in strawberry showing malformation of the fruits together with the typical green petals symptoms was detected in some North Western Italy cultivations. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rDNA and GroEL sequences specific for phytoplasmas. Bands of 1.2 kb were obtained in both cases after nested-PCR assays and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows subgroup 16SrI-C, the GroELI grouping confirm all the strains from strawberry to be identical to each other and to GroELI-VI group. This is the first multigene molecular identification of strawberry green petals phytoplasmas in Italy.
2012
Identification and GroEL gene characterization of green petal phytoplasma infecting strawberry in Italy / Contaldo N.; J.F. Mejia; S. Paltrinieri; A. Calari; A. Bertaccini. - In: PHYTOPATHOGENIC MOLLICUTES. - ISSN 2249-4669. - STAMPA. - 2:2(2012), pp. 59-62. [10.5958/j.2249-4669.2.2.009]
Contaldo N.; J.F. Mejia; S. Paltrinieri; A. Calari; A. Bertaccini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/139037
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