Purpose The aim of this study is to explore the feasibility of 11C-Choline PET in the assessment of the degree of inflammation in the Chlamydia muridarum genital infection model. Procedures Forty female Balb/c mice received 2.5 mg of medroxyprogesterone acetate i.m. 9 and 2 days prior to the infection: 21 mice were infected by C. muridarum into the vaginal vault, 12 mice were treated with inactivated chlamydiae, and 7 mice were SPG buffer-treated as negative controls. Three healthy control mice were not treated with progesterone. Mice in each category were randomly subdivided in two groups: (1) sacrificed at 5, 10, 15, and 20 days for histological analysis and (2) undergoing 11C-Choline PET at days 5, 10, and 20 post-infection (20 MBq of 11C-Choline, uptake time of 10 min, acquisition through a small-animal PET tomograph for 15 min). Results Infected animals showed a significantly higher standardized uptake value than both controls and animals inoculated with heat-inactivated chlamydiae in each PET scan (P < 0.05). All organs of the infected animals had scores of inflammation ranging between 2 and 3 at day 5, decreasing to 1–2 at day 20. Conclusions This preliminary result demonstrated that 11C-Choline PET can highlight a specific proliferation mechanism of inflammatory cells induced by C. muridarum, thanks to a very high sensitivity in detecting very small amounts of tracer in inflammatory cells.
Usefulness of (11)C-Choline Positron Emission Tomography for Genital Chlamydial Infection Assessment in a Balb/c Murine Model / Marangoni A.; Nanni C.; Quarta C.; Foschi C.; Russo I.; Nardini P.; D'Errico A.; Rosini F.; Ferretti A.; Aldini R.; Cevenini R.; Rubello D.. - In: MOLECULAR IMAGING AND BIOLOGY. - ISSN 1536-1632. - STAMPA. - 15:(2013), pp. 450-455. [10.1007/s11307-013-0612-4]
Usefulness of (11)C-Choline Positron Emission Tomography for Genital Chlamydial Infection Assessment in a Balb/c Murine Model.
MARANGONI, ANTONELLA;FOSCHI, CLAUDIO;D'ERRICO, ANTONIETTA;ALDINI, RITA;CEVENINI, ROBERTO;
2013
Abstract
Purpose The aim of this study is to explore the feasibility of 11C-Choline PET in the assessment of the degree of inflammation in the Chlamydia muridarum genital infection model. Procedures Forty female Balb/c mice received 2.5 mg of medroxyprogesterone acetate i.m. 9 and 2 days prior to the infection: 21 mice were infected by C. muridarum into the vaginal vault, 12 mice were treated with inactivated chlamydiae, and 7 mice were SPG buffer-treated as negative controls. Three healthy control mice were not treated with progesterone. Mice in each category were randomly subdivided in two groups: (1) sacrificed at 5, 10, 15, and 20 days for histological analysis and (2) undergoing 11C-Choline PET at days 5, 10, and 20 post-infection (20 MBq of 11C-Choline, uptake time of 10 min, acquisition through a small-animal PET tomograph for 15 min). Results Infected animals showed a significantly higher standardized uptake value than both controls and animals inoculated with heat-inactivated chlamydiae in each PET scan (P < 0.05). All organs of the infected animals had scores of inflammation ranging between 2 and 3 at day 5, decreasing to 1–2 at day 20. Conclusions This preliminary result demonstrated that 11C-Choline PET can highlight a specific proliferation mechanism of inflammatory cells induced by C. muridarum, thanks to a very high sensitivity in detecting very small amounts of tracer in inflammatory cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.