Live vaccines predominantly control avian metapneumovirus (AMPV) infection in the poultry industry but these can persist after application and revert to virulence. Sequencing of the G gene of the dominant subtype B VCO3 vaccinal strain identified a unique nucleotide substitution (A→G, nucleotide 91) which fortuitously introduced an MseI restriction endonuclease site within the amplicon obtained from a popular AMPV diagnostic RT- nested PCR. An Msel digestion protocol was developed then validated using Italian B subtype viruses of known field virus or vaccine origin.
RAPID DIFFERENTIATION OF VACCINE AND FIELD STRAINS OF AVIAN METAPNEUMOVIRUS OF SUBTYPE B BY RESTRICTION ENZYME DIGESTION OF PCR PRODUCTS
LISTORTI, VALERIA;LUPINI, CATERINA;CATELLI, ELENA
2012
Abstract
Live vaccines predominantly control avian metapneumovirus (AMPV) infection in the poultry industry but these can persist after application and revert to virulence. Sequencing of the G gene of the dominant subtype B VCO3 vaccinal strain identified a unique nucleotide substitution (A→G, nucleotide 91) which fortuitously introduced an MseI restriction endonuclease site within the amplicon obtained from a popular AMPV diagnostic RT- nested PCR. An Msel digestion protocol was developed then validated using Italian B subtype viruses of known field virus or vaccine origin.File in questo prodotto:
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