A novel nanocarrier system was designed and developed with key components uniquely structured at the nanoscale for the early cancer diagnosis and treatment. In order to perform magnetic resonance imaging (MRI), hydrophilic superparamagnetic maghemite nanoparticles (NPs) were synthesized and coated with a lipophilic organic ligand. Next, they have been entrapped into polymeric NPs made of biodegradable poly(lactic-co-glycolic acid) (PLGA) linked to polyethylene glycol (PEG). In addition, resulting NPs have been conjugated on their surface with a 2,2'-(7-(4-((2-aminoethyl)amino)-1-carboxy-4-oxobutyl)-1,4,7-triazonane-1,4-diyl)diacetic acid (NODA) ligand for subsequent 68Ga incorporation. A cell-based cytotoxicity assay has been employed to verify the in vitro cell viability of human pancreatic cancer cells exposed to this nanosystem. Finally, in vivo positron emission tomography-computerized tomography (PET-CT) biodistribution studies in healthy animals were performed.
M. Comes franchini, E. Locatelli, L. Passoni, L. L. Israel, M. Naddaka, A. Pucci, et al. (2012). Biocompatible nanocomposite for PET/MRI hybrid imaging of pancreatic cancer. INTERNATIONAL JOURNAL OF NANOMEDICINE, 7, 6021-6033 [10.2147/IJN.S38107].
Biocompatible nanocomposite for PET/MRI hybrid imaging of pancreatic cancer
COMES FRANCHINI, MAURO;LOCATELLI, ERICA;
2012
Abstract
A novel nanocarrier system was designed and developed with key components uniquely structured at the nanoscale for the early cancer diagnosis and treatment. In order to perform magnetic resonance imaging (MRI), hydrophilic superparamagnetic maghemite nanoparticles (NPs) were synthesized and coated with a lipophilic organic ligand. Next, they have been entrapped into polymeric NPs made of biodegradable poly(lactic-co-glycolic acid) (PLGA) linked to polyethylene glycol (PEG). In addition, resulting NPs have been conjugated on their surface with a 2,2'-(7-(4-((2-aminoethyl)amino)-1-carboxy-4-oxobutyl)-1,4,7-triazonane-1,4-diyl)diacetic acid (NODA) ligand for subsequent 68Ga incorporation. A cell-based cytotoxicity assay has been employed to verify the in vitro cell viability of human pancreatic cancer cells exposed to this nanosystem. Finally, in vivo positron emission tomography-computerized tomography (PET-CT) biodistribution studies in healthy animals were performed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
