Recently, label-free techniques are developing in which the presence of the analyte macromolecule itself can induce detectable physico-chemical changes. The struggle is to make such techniques sufficiently sensitive and specific. In our work, we are trying to implement DNA-based surface-bound amplification strategies that can serve to amplify the read-out signal of DNA hybridization in a label-free biosensor, such as those measuring the electrochemical properties of an electrode interface.
Surface-bound DNA self-assembly or enzymatic reactions toward the PCR-free amplification of nucleic acids biosensor signals / G Zuccheri; A Vinelli; M Onofri; B Samorì. - In: EUROPEAN CELLS & MATERIALS. - ISSN 1473-2262. - STAMPA. - 20 (Supp. 3):(2010), pp. 285-285.
Surface-bound DNA self-assembly or enzymatic reactions toward the PCR-free amplification of nucleic acids biosensor signals
ZUCCHERI, GIAMPAOLO;VINELLI, ALESSANDRA;ONOFRI, MANUELE;
2010
Abstract
Recently, label-free techniques are developing in which the presence of the analyte macromolecule itself can induce detectable physico-chemical changes. The struggle is to make such techniques sufficiently sensitive and specific. In our work, we are trying to implement DNA-based surface-bound amplification strategies that can serve to amplify the read-out signal of DNA hybridization in a label-free biosensor, such as those measuring the electrochemical properties of an electrode interface.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.