A new amperometric immunosensor for 2,4,6- trinitrotoluene based on the working principle of competitive enzyme-linked immunosorbent assay was developed and characterised. An electrodeposited nanogold substrate was functionalised by deposition of self-assembled monolayers of 2-aminoethanethiol as linkers for the subsequent immobilisation of polyamidoaminic dendrimers. Our approach makes use of those dendrimers to anchor a trinitrobenzene-ovalbumin conjugate on the electrode surface. The immunosensor was tested and validated for the determination of 2,4,6-trinitrotoluene showing high selectivity with respect to other nitroaromatic compounds, a limit of detection of 4.8 ng/mL and a limit of quantitation of 6 ng/mL. The immunosensor was tested for the quantification of the analyte in spiked soils and in a real sample of post-blast soil, evidencing a good recovery rate (113 %).

M.Giannetto, E.Maiolini, E.Nora Ferri, S.Girotti, G.Mori, M.Careri (2013). Competitive Amperometric Immunosensor Based on Covalent Linking of Haptens to Dendrimer-functionalized Nanogold Substrate for the Determination of 2,4,6-trinitrotoluene. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 405(2-3), 737-743 [10.1007/s00216-012-6137-x].

Competitive Amperometric Immunosensor Based on Covalent Linking of Haptens to Dendrimer-functionalized Nanogold Substrate for the Determination of 2,4,6-trinitrotoluene

MAIOLINI, ELISABETTA;FERRI, ELIDA NORA;GIROTTI, STEFANO;
2013

Abstract

A new amperometric immunosensor for 2,4,6- trinitrotoluene based on the working principle of competitive enzyme-linked immunosorbent assay was developed and characterised. An electrodeposited nanogold substrate was functionalised by deposition of self-assembled monolayers of 2-aminoethanethiol as linkers for the subsequent immobilisation of polyamidoaminic dendrimers. Our approach makes use of those dendrimers to anchor a trinitrobenzene-ovalbumin conjugate on the electrode surface. The immunosensor was tested and validated for the determination of 2,4,6-trinitrotoluene showing high selectivity with respect to other nitroaromatic compounds, a limit of detection of 4.8 ng/mL and a limit of quantitation of 6 ng/mL. The immunosensor was tested for the quantification of the analyte in spiked soils and in a real sample of post-blast soil, evidencing a good recovery rate (113 %).
2013
M.Giannetto, E.Maiolini, E.Nora Ferri, S.Girotti, G.Mori, M.Careri (2013). Competitive Amperometric Immunosensor Based on Covalent Linking of Haptens to Dendrimer-functionalized Nanogold Substrate for the Determination of 2,4,6-trinitrotoluene. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 405(2-3), 737-743 [10.1007/s00216-012-6137-x].
M.Giannetto; E.Maiolini; E.Nora Ferri; S.Girotti; G.Mori; M.Careri
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/132695
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