In the present study, a monoclonal antibody (mAb), D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. Negevensis-infected cells, by using the immunofluorescence test. D5-14 mAb, reacting in immunoblot with an approximately 64-66kDa protein of C. trachomatis LGV2 serotype, recognized a protein with the same molecular mass, when tested with S. negevensis elementary bodies.
Donati M., Di Paolo M., Avanzi S., Di Francesco A., Fiani N., Cevenini R. (2013). Detection of Simkania negevensis in cell culture by using a monoclonal antibody. NEW MICROBIOLOGICA, 36, 85-88.
Detection of Simkania negevensis in cell culture by using a monoclonal antibody
DONATI, MANUELA;AVANZI, SIMONE;DI FRANCESCO, ANTONIETTA;CEVENINI, ROBERTO
2013
Abstract
In the present study, a monoclonal antibody (mAb), D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. Negevensis-infected cells, by using the immunofluorescence test. D5-14 mAb, reacting in immunoblot with an approximately 64-66kDa protein of C. trachomatis LGV2 serotype, recognized a protein with the same molecular mass, when tested with S. negevensis elementary bodies.File in questo prodotto:
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