ABSTRACT PURPOSE. Despite improvements in operative environment and surgical techniques, post-operative infections remain one of the most devastating complications in total joint replacement prostheses. Several efforts have been addressed to modify the surface of materials, in order to prevent bacterial adhesion and colonization. Here, we show a one-pot electrochemical surface modification process for co-deposition of calcium phosphate and gentamicin, with the aim to trigger specific biological responses and impart antibacterial properties on titanium-based devices. METHODS. Gentamicin-loaded Calcium Phosphate coatings were deposited on Ti specimens via cathodic polarization in an electrochemical bath containing different amount of the antibiotic salt (1-10 mg mL-1). Coatings were evaluated in terms of chemico-physical properties, via SEM/EDX, XRD, and ICP analysis, and antibacterial activity, via agar disc diffusion tests on S. aureus 8325-4 and SA113. RESULTS. An effective incorporation of gentamicin in brushite coatings, without any major effect on coating morphology, has been obtained. Gentamicin-loaded coatings showed an antibacterial efficacy on both S. aureus strains, with a tunable dose-dependant activity. CONCLUSION. Electrochemical deposition can be advantageously a simple and powerful method to obtain bone contact surface coatings with tailored antibacterial properties.
Electrochemically deposited gentamicin-loaded calcium phosphate coatings for bone tissue integration
ARCIOLA, CARLA RENATA;
2012
Abstract
ABSTRACT PURPOSE. Despite improvements in operative environment and surgical techniques, post-operative infections remain one of the most devastating complications in total joint replacement prostheses. Several efforts have been addressed to modify the surface of materials, in order to prevent bacterial adhesion and colonization. Here, we show a one-pot electrochemical surface modification process for co-deposition of calcium phosphate and gentamicin, with the aim to trigger specific biological responses and impart antibacterial properties on titanium-based devices. METHODS. Gentamicin-loaded Calcium Phosphate coatings were deposited on Ti specimens via cathodic polarization in an electrochemical bath containing different amount of the antibiotic salt (1-10 mg mL-1). Coatings were evaluated in terms of chemico-physical properties, via SEM/EDX, XRD, and ICP analysis, and antibacterial activity, via agar disc diffusion tests on S. aureus 8325-4 and SA113. RESULTS. An effective incorporation of gentamicin in brushite coatings, without any major effect on coating morphology, has been obtained. Gentamicin-loaded coatings showed an antibacterial efficacy on both S. aureus strains, with a tunable dose-dependant activity. CONCLUSION. Electrochemical deposition can be advantageously a simple and powerful method to obtain bone contact surface coatings with tailored antibacterial properties.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
