CRIS Current Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishAims: The induction of apoptosis in the human enterocyte-like Caco-2 cell line mediated by three Bifidobacterium longum strains, which displays different adhesion properties, was investigated. Methods and Results: The three strains tested were defined for their adhesion properties using adhesion and autoaggregation assays. Strain B12 shows strong autoaggregation and strong adherence to Caco-2 cells; strain B18 shows strong autoaggregation and intermediate adherence; strain B2990 shows poor autoaggregation and poor adherence. Cocultures of Caco-2 cells with B. longum resulted in the induction of apoptosis, determined by DNA fragmentation test, only when autoaggregating or strongly adherent strains were used (B12 and B18). Conclusion: These results indicate that the interaction with aggregating/adherent probiotics can induce apoptosis of human colon epithelial cells, suggesting a role for these bacteria in the regulation of the cellular homeostasis of the gastrointestinal tract. Significance and Impact of the Study: B. longum as a probiotic is known for his beneficial effects to the human gut; the knowledge of its immunomodulatory and antitumor activities can be crucial for the healthcare and the prevention of cancer.
| Titolo: | Role of Bifidobacterium longum in the induction of apoptosis in the human enterocyte-like Caco-2 cell line |
| Autore/i: | NISSEN, LORENZO; BAFFONI, LOREDANA; PASINI, LUCA; BIAVATI, BRUNO; MALAGOLINI, NADIA; DELLA VALLE, GIULIANO; SGORBATI, BARBARA |
| Autore/i Unibo: | |
| Anno: | 2005 |
| Titolo del libro: | Atti Biofutura 2005 |
| Pagina iniziale: | 39 |
| Abstract: | Aims: The induction of apoptosis in the human enterocyte-like Caco-2 cell line mediated by three Bifidobacterium longum strains, which displays different adhesion properties, was investigated. Methods and Results: The three strains tested were defined for their adhesion properties using adhesion and autoaggregation assays. Strain B12 shows strong autoaggregation and strong adherence to Caco-2 cells; strain B18 shows strong autoaggregation and intermediate adherence; strain B2990 shows poor autoaggregation and poor adherence. Cocultures of Caco-2 cells with B. longum resulted in the induction of apoptosis, determined by DNA fragmentation test, only when autoaggregating or strongly adherent strains were used (B12 and B18). Conclusion: These results indicate that the interaction with aggregating/adherent probiotics can induce apoptosis of human colon epithelial cells, suggesting a role for these bacteria in the regulation of the cellular homeostasis of the gastrointestinal tract. Significance and Impact of the Study: B. longum as a probiotic is known for his beneficial effects to the human gut; the knowledge of its immunomodulatory and antitumor activities can be crucial for the healthcare and the prevention of cancer. |
| Data prodotto definitivo in UGOV: | 27-feb-2006 |
| Appare nelle tipologie: | 4.02 Riassunto (Abstract) |
File in questo prodotto:
Copyright © 2021